Effect of loop on the stability of intramolecular triplex DNA

• Published in: Science China. Chemistry Vol. 40; no. 6; pp. 650 - 656
• Main Author: 杨林静 白春礼 李任植 汪新文 王琛
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01.12.1997
• Subjects: DNA; intra-triplex; loop; mechanics; molecular; stability
• ISSN: 1674-7291; 1006-9291; 1869-1870; 1862-2771
• DOI: 10.1007/BF02875484

Conformation stabilities of intramolecular triple-helical DNAs (intra-tnplex DNAs: 5-d(TC)6-d(T)m-d(CT)6-d(C)n-d(AG)6-3’,where m and n are chosen to be 4 and 3) have been examined by molecular mechanics.The four intra-triplexes (H-DNAs) are compared with the mtermolecular triplexes d(TC)6 * d ( AG)6 d(CT)6.The results revealed that loops do not significantly influence the mtratriplex conformations,loop conformations,however,depend partly on its length.Loop also makes strand Ⅱ of every intra-triplex DNA longer than that of the inter-triplex DNA.Most of residue sugar conformations of triple-helical DNAs are S-type,there aiso exist,however,N-type conformation and the conformations between S-type and N-type Possible models of the five triplex DNAs are presented.