Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 26; no. 5; pp. 524 - 527
• Main Author: 张建初 夏蕾 张晓菊 杨卫兵 白明
• Format: Journal Article
• Language: English
• Published: China Department of Pulmonary Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China%Department of Pulmonary Medicine, Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, China 2006
• Subjects: Animals; Arginine - pharmacology; Arginine - therapeutic use; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Female; L-精氨酸; Lung - enzymology; Male; Nitric Oxide Synthase Type III - genetics; Nitric Oxide Synthase Type III - metabolism; Pulmonary Embolism - drug therapy; Pulmonary Embolism - enzymology; Rats; Rats, Wistar; RNA, Messenger - genetics; RNA, Messenger - metabolism; 基因表达; 肺血管栓塞; cyclooxygenase2; pulmonary thromboembolism; nitric oxide synthase; L-arginine
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-006-0509-4

The influence of L-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and L-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, L-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism.