Phase-sensitive dynamic micro-optical coherence tomography for high-speed intracellular motion imaging

Dynamic optical coherence tomography with micrometer resolution (DµOCT) offers enhanced contrast information by evaluating time-dependent signal fluctuations in images of living tissue. DµOCT's use has been limited to imaging excised fresh tissue specimens or 3D cell cultures due to the long ob...

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Bibliographic Details
Published inOptics letters Vol. 50; no. 15; p. 4734
Main Authors Schulz-Hildebrandt, Hinnerk, Gardecki, Joseph A, Miller, Tiffany, Avila-Wallace, Maria, Villareyna-Lopez, Erica, Tearney, Guillermo
Format Journal Article
LanguageEnglish
Published United States 01.08.2025
Subjects
Algorithms
Animals
Humans
Intracellular Space - metabolism
Motion
Movement
Time Factors
Tomography, Optical Coherence - methods
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ISSN1539-4794
DOI10.1364/OL.563024

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Summary:Dynamic optical coherence tomography with micrometer resolution (DµOCT) offers enhanced contrast information by evaluating time-dependent signal fluctuations in images of living tissue. DµOCT's use has been limited to imaging excised fresh tissue specimens or 3D cell cultures due to the long observation times required, typically ranging from 1.35 to 25 seconds. To reduce the time needed to obtain DµOCT images, we developed a phase-based algorithm that analyzes intracellular motion by measuring phase changes between adjacent B-scans. This approach significantly reduces imaging time to as low as 40.5 ms while providing a quantitative measure of intracellular motion.
ISSN:1539-4794
DOI:10.1364/OL.563024