Protective effect of butanol extracts of skin of Anguilla japonica against endoplasmic reticulum stress-induced insulin resistance via the AMPK pathway in L6 myotubes

• Published in: Food science and biotechnology Vol. 24; no. 3; pp. 1069 - 1075
• Main Authors: Na, Min Kyun, Jeong, Yong-Tae, Li, Xian, Jin, Fansi, Hwang, Seung-Lark, Kim, Geum Jin, Yang, Ju Hye, Chang, Young-Chae, Kim, Dong Soo, Kim, Cheorl-Ho, Chang, Hyeun Wook
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.06.2015; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Food Science; Nutrition; Research Article; 식품과학; skin; Insulin resistance; AMP-activated protein kinase; L6 myotube
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0137-5

The effect of butanol extracts of the skin of Anguilla japonica (BESA) on endoplasmic reticulum (ER) stress-induced insulin resistance in L6 myotubes was investigated. Western blotting revealed that BESA increased phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase, and stimulated glucose uptake in L6 myotubes. Stimulation of AMPK and glucose uptake by BESA were significantly ( p <0.05) reduced by siRNA LKB1 or siRNA AMPK, compared with controls, suggesting that enhanced glucose uptake by BESA was predominantly accomplished via an LKB1-mediated AMPK activation pathway. In addition, BESA effectively reduced phosphorylation of ER stress markers, RNA-activated protein kinase-like ER resident kinase, JNK, and significantly ( p <0.01) increased glucose uptake via AMPK activation in tunicamycin-treated L6 myotubes, compared with controls. Improvement of insulin sensitivity under ER stress conditions by BESA is predominantly accomplished via an LKB1-AMPK-dependent pathway.