Potential mechanism of kaempferol against Cu²+-induced oxidative stress through chelating activity and regulation of nuclear factorerythroid-2-related factor 2 signaling

• Published in: Food science and biotechnology Vol. 21; no. 5; pp. 1469 - 1475
• Main Authors: Kim, G.N., Kyungnam University, Changwon, Republic of Korea, Kim, E.S., Hannam University, Daejeon, Republic of Korea, Kwon, Y.I., Hannam University, Daejeon, Republic of Korea, Jang, H.D., Hannam University, Daejeon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.10.2012; 한국식품과학회
• Subjects: cellular antioxidant activity; Chemistry; Chemistry and Materials Science; Food Science; heme oxygenase-1; KAEMPFEROL; nuclear factor-erythroid-2-related factor 2; Nutrition; Research Article; 식품과학; cellular antioxidant activity; nuclear factor-erythroid-2-related factor 2; Cu; heme oxygenase-1; kaempferol; chelating activity
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-012-0194-y

Metal ions play important roles in various biological processes of living systems. However, the generation of reactive oxygen species (ROS) is also closely linked with the participation of redox-active metal ions such as copper, iron, chromium, and cobalt ions. Excessive production of ROS by redox-active metal ions can cause oxidative stress and further oxidative stress-related diseases. This study shows the results of the antioxidant activity of kaempferol in both ORAC∧OH center dot and Cu²+-treated HepG2 cells. Preventive mechanism of kaempferol in Cu²+-treated HepG2 cells is also elucidated. These results suggest that both cellular Cu²+-chelating activity and expression of phase Ⅱ detoxifying enzymes such as HO-1 and GSTA2 through activating Nrf2 are required for cellular antioxidant activity of kaempferol in Cu²+-treated HepG2 cells. Our findings provide the scientific evidence for the development of Nrf2 targeting dietary antioxidant to prevent oxidative stress-related conditions.