Molecular Weight Determination of Chitosan with Antibacterial Activity Using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry Analysis

• Published in: Macromolecular research Vol. 30; no. 2; pp. 90 - 98
• Main Authors: Lee, Yong Hyun, Park, So Yeon, Hwang, You Jin, Park, Jae Kweon
• Format: Journal Article
• Language: English
• Published: Seoul The Polymer Society of Korea 01.02.2022; Springer Nature B.V; 한국고분자학회
• Subjects: Antimicrobial agents; Biological activity; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chitosan; Complex Fluids and Microfluidics; Desorption; Hydrolysates; Ionization; Ions; Mass spectrometry; Microorganisms; Molecular weight; Nanochemistry; Nanotechnology; Physical Chemistry; Polymer Sciences; Scientific imaging; Soft and Granular Matter; Spectroscopy; 고분자공학; fine dust; chitosan; antibacterial effect; 1,3-butylene glycol; molecular weight
• ISSN: 1598-5032; 2092-7673
• DOI: 10.1007/s13233-022-0013-0

The antibacterial effect of chitosan with different molecular weights was investigated to define their potent biological activity. Both chitosan hydrolysates designated to CTSN-P30 and CTSN-B8 derived from the hydrolysis of high molecular weight chitosan showed significant synergistic antibacterial effects toward Staphylococcus aureus in the presence of 1,3-butylene glycol. The concentration-dependent antimicrobial effect is very important in determining the minimum inhibitory concentration (MIC) of both CTSN-P30 and CTSN-B8. Each MIC 50 of CTSN-P30 and CTSN-B8 was determined to be about 25 and 50 µg/mL, respectively. Among chitosan hydrolysates, CTSN-P30 can effectively protect the membrane from the penetrating of fine dust which may contain unspecified microorganisms. Also, CTSN-P30, which showed very significant antimicrobial activity, was confirmed to be a hydrolysate containing (GlcNAc) 3 , and other hydrolysates consisting of (GlcN) 5 to (GlcN) 15 using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis. These results demonstrated the feasibility of CTSN-P30 and 1,3-butylene glycol to use as a potent antibacterial and find a dustproof agent for the preparation of various industrial products.