Reg Ⅳ, a differentially expressed gene in colorectal adenoma

• Published in: Chinese medical journal Vol. 116; no. 6; pp. 918 - 922
• Main Author: 张宇伟 来茂德 谷雪梅 罗敏捷 邵丽娜
• Format: Journal Article
• Language: English
• Published: China Department of Pathology, School of Medicine of Zhejiang University, Hangzhou 310006, China 01.06.2003
• Subjects: Adenoma - genetics; Adenoma - metabolism; Blotting, Northern; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Humans; Lectins, C-Type - biosynthesis; Lectins, C-Type - genetics; Nucleic Acid Hybridization; Pancreatitis-Associated Proteins; Prognosis; Reg; Reverse Transcriptase Polymerase Chain Reaction; 基因表达; 结肠直肠肿瘤; adenoma; Reg; colorectal neoplasms; bioinformatics
• ISSN: 0366-6999; 2542-5641

Objective To discover and identify differentially expressed genes associated with colorectal adenomaforma tion and the role of ReglV in colorectal adenoma differentiation.Methods A subtracted cDNA library was constructed with cDNAs that were isolated from either the normal mucosa or adenoma tissue of a single patient. Suppressive subtractive hybridization (SSH)combined with virtual northern blotting was used to characterize differentially expressed genes and contigs were assembled by electronic cloning (in silico cloning ) with the EST database. Semi-quantitative RT-PCR was performed in 9 colorectal adenomas.Results The amino acid sequence was determined with open reading frame (ORF) prediction software and was found to be 100% homologous to the protein product of Reg Ⅳ (a novel gene isolated from a large inflammatory bowel disease library). RegⅣ was found to be highly expressed in all of the adenoma samples (9/9) compared with the normal mucosa samples, while 5/6 casess howed RegⅣ to be more strongly expressed in adenocarcinoma.Conclusion RegⅣ may play an important role in the initiation of colorectal adenoma differentiation,and its detection mav be useful in the earlv diaonosis of colorectal adenoma formation.