Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

• Published in: Journal of Zhejiang University. A. Science Vol. 5; no. 4; pp. 467 - 471
• Main Author: 陈建忠 朱海红 刘克洲 陈智
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.04.2004
• Subjects: Animals; Antibodies; Antigens; Cytokines; Cytokines - biosynthesis; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA vaccines; DNA疫苗; Enzyme-linked immunosorbent assay; HBV; Hepatitis B; Hepatitis B Antibodies - blood; Hepatitis B Antigens - genetics; Hepatitis B surface antigen; Hepatitis B Vaccines - administration & dosage; Hepatitis B Vaccines - genetics; Hepatitis B Vaccines - immunology; Hepatitis B virus - genetics; Hepatitis B virus - immunology; Immune response; Immune response (cell-mediated); Immune system; Immunity, Cellular; Immunization; In Vitro Techniques; Interleukin 18; Interleukin 4; Interleukin-18 - administration & dosage; Interleukins; Lymphocytes T; M gene; Mice; Mice, Inbred BALB C; Recombinant Proteins - administration & dosage; Splenocytes; T-Lymphocytes, Cytotoxic - immunology; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Viral Matrix Proteins - genetics; Viral Matrix Proteins - immunology; γ-Interferon; 乙型肝炎病毒; 白细胞间介素; 细胞免疫响应
• ISSN: 1009-3095; 1673-565X; 1862-1775
• DOI: 10.1631/jzus.2004.0467

Objective:To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines.Methods:BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA;splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro.Results:The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone,but there was not significantly different (P>0.05).Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05).Conclusion:The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.