Effect of Exercise on Urinary Levels of 8-Hydroxy-2'-deoxyguanosine (8-OHdG)

• Published in: Kita Kantō igaku (The Kitakanto Medical Journal) Vol. 52; no. 5; pp. 351 - 356
• Main Authors: Toda, Takuma, Yamazaki, Tohru, Handa, Hiroshi, Arakawa, Chiaki, Murakami, Hirokazu, Fukuda, Harue, Morita, Kimio, Ogawara, Hatsue, Sakurai, Hitomi, Tomiyosi, Satoko
• Format: Journal Article
• Language: English; Japanese
• Published: The Kitakanto Medical Society 2002
• Subjects: 8-hydroxy-2'-deoxyguanosine (8-OHdG); ergometer exercise; oxidative stress; urine
• ISSN: 1343-2826; 1881-1191; 1881-1191
• DOI: 10.2974/kmj.52.351

Background and Aim : Oxidative DNA stress is related to the development of various diseases. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidative DNA damage produced by specific enzymatic cleavage processes and is a popular marker for the evaluation of oxidative DNA damage. We investigated various factors influencing the excretion of 8-OHdG in the urine of 35 healthy volunteers. Materials and Methods : Urine samples were collected from 35 healthy volunteers between the age of 20 and 34 (mean age, 22.2±3.0 years male : female ratio, 23:12) and stored at -80°C until analysis. The 8-OHdG levels were analyzed using a competitive ELISA kit. Multistage physical exercise was performed using a bicycle ergometer and continued until the subjects felt that the exercise was “very, very hard” (Borg Index 19). Results : The mean level of urinary 8-OHdG corrected for the creatinine (Cr) concentration (μg/g Cr) was 12.0±3.5μg/g Cr (n=35). The mean level of 8-OHdG, corrected for subject weight (kg) and unit time (ng/kg/h), was 13.3±7.2 ng/kg/h. The mean levels of 8-OHdG (ng/kg/h) varied slightly with the sampling time. The mean level of 8-OHdG (ng/kg/h) at 9 : 00 tended to be higher than the levels at 12:00, 15:00, and 18:00 (not statistically different). The level of 8-OHdG (μg/g Cr) was not affected by the sampling time. No significant differences were observed between smokers and non-smokers or males and females. The mean level of 8-OHdG (μg/g Cr) after exercise was significantly elevated, compared with before exercise and the level during the recovery phase. Conclusions : Multistage ergometer exercises caused oxidative DNA damage in healthy volunteers.