Asparagine-linked sugar chains of membrane glycoproteins from healthy and Wiskott-Aldrich syndrome patient's erythrocytes

• Published in: Japanese Journal of Clinical Immunology Vol. 9; no. 4; pp. 261 - 264
• Main Authors: Orii, Tadao, Hirano, Yukiko, Kondo, Naomi, Motoyoshi, Fumiaki
• Format: Journal Article
• Language: English
• Published: The Japan Society for Clinical Immunology 1986
• ISSN: 0911-4300; 1349-7413
• DOI: 10.2177/jsci.9.261

Asparagine-linked sugar chains of membrane glycoproteins from healthy and Wiskott-Aldrich syndrome patient's (WAS) erythrocytes were investigated. Membrane fractions were prepared by the hypotonic shock and the centrifugation. Analysis of the asparagine-linked sugar chains of glycoproteins was carried out by the chromatography and the sequential exoglycosidase digestion. In results, the main oligosaccaride in asparagine-linked sugar chains of the membrane of erythrocytes from healthy and WAS were biantennary sugar chain with bisect N-acetyl-glucosamine (Gal2•GlcNAc2•Man3•GlcNAc•GlcNAc•Fuc•GlcNAcOT) respectively. Moreover, there was biantennary sugar chain with fucose, but was not biantennary sugar chain without fucose, in both erythrocytes membrane.