Determination of Antibacterial Agents for Animals in Swine Muscles by Microbiological Screening and LC-MS/MS

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 61; no. 4; pp. 109 - 118
• Main Authors: Nagano, Chieko, Yoshikawa, Souichi, Hashimoto, Tsuneo, Matsushima, Yoko, Sasamoto, Takeo, Sekimura, Kotaro, Hayashi, Hiroshi, Koike, Hiroshi, Ohba, Yumi, Hayashi, Momoka, Otsuka, Kenji, Kanda, Maki
• Format: Journal Article
• Language: Japanese
• Published: Tokyo Japanese Society for Food Hygiene and Safety 25.08.2020; Japan Science and Technology Agency
• Subjects: Acetonitrile; Animals; Antibacterial agents; antibacterial agents for animal; Antibiotics; Antiinfectives and antibacterials; Cartridges; Drugs; LC-MS/MS; Liquid chromatography; Mass spectrometry; Mass spectroscopy; microbiological screening; Muscles; pH; pH effects; Screening; Swine; swine muscle
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.61.109

The determination of antibacterial agents for animals in swine muscles was improved by microbiological screening and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. In the first instance, the residual drugs were extracted from the samples using the Na2EDTA-McIlvaine buffer (pH 6.0). Subsequently, the agents were purified utilizing a PLS-3 cartridge and extracted with acetonitrile. Considering the microbiological methods, the sensitivities of the investigated drugs were higher and the test plate conditions were improved using a new reference organism Geobacillus stearothermophilus. As a result, a microbiological screening approach able to detect 33 drugs at MRL was developed in Japan. Remarkable, no false positives were detected. Moreover, the same preparation method enabled rapid and reliable microbiological screening, resulting in efficient screening with no undeterminable results.