Co-regulation of dopamine D1 receptor and uncoupling protein-2 expression in 3-nitropropionic acid-induced neurotoxicity: Neuroprotective role of l-carnitine

• Published in: Neuroscience letters Vol. 410; no. 1; pp. 62 - 65
• Main Authors: Binienda, Zbigniew K., Ali, Syed F., Virmani, Ashraf, Amato, Antonino, Salem, Nadia, Przybyla, Beata D.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier 13.12.2006
• Subjects: Animals; Biological and medical sciences; Body Temperature - drug effects; Carnitine - therapeutic use; Disease Models, Animal; Drug Interactions; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Ion Channels - genetics; Ion Channels - metabolism; Male; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Neostriatum - drug effects; Neostriatum - metabolism; Neuroprotective Agents - therapeutic use; Neurotoxicity Syndromes - etiology; Neurotoxicity Syndromes - metabolism; Neurotoxicity Syndromes - prevention & control; Neurotoxins - toxicity; Nitro Compounds; Propionates; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D1 - genetics; Receptors, Dopamine D1 - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Uncoupling Protein 2; Vertebrates: nervous system and sense organs; Neuroprotection; Dopamine; Rat; Rodentia; Central nervous system; D1 Dopamine receptor; Basal ganglion; Corpus striatum; Catecholamine; Protein; Encephalon; Carnitine; Uncoupling proteins; Striatum; Vertebrata; Neurotoxicity; Mammalia; Animal; 3-Nitropropionic acid; Neurotransmitter; L-Carnitine
• ISSN: 0304-3940
• DOI: 10.1016/j.neulet.2006.09.070

This study tested the hypothesis that the expression of uncoupling proteins (UCPs) and dopamine (DA) system genes is responsive to 3-nitropropionic acid (3-NPA) neurotoxic effects and to the neuroprotective effects of the mitochondrial enhancer, L-carnitine (LC), in the rat striatum. Inactivation of mitochondrial succinate dehydrogenase (SDH) by 3-NPA results in hypoxic brain damage. Hypoxic conditions induce uncoupling protein-2 (UCP-2). An increase in UCP-2 expression may lead to a decrease in production of reactive oxygen species (ROS) associated with energy depletion. However, this adaptive response can also lead to a reduction of ATP that may further contribute to energy deficit and mitochondrial dysfunction. Here, male adult Sprague-Dawley rats (n=5/group) were injected either with saline or 3-NPA at 30 mg/kg, s.c. alone or 30 min after pre-treatment with LC (100mg/kg, i.p.). Rectal temperature was monitored before treatment and 4h following 3-NPA administration. Animals were sacrificed 4h post-treatment. Total RNA was isolated from the striatum and transcripts of UCP-2, UCP-4 and UCP-5 genes, as well as genes related to dopamine metabolism, such as DA D(1) and D(2) receptors, tyrosine hydroxylase (TH), monoamine oxidase-B (MAO-B), and vesicular monoamine transporter-2 (VMAT-2), were measured using real-time reverse transcription polymerase chain reaction (RT-PCR). While core temperature decreased significantly in 3-NPA-treated rats, LC significantly inhibited the hypothermic effect of 3-NPA (p<0.05). 3-NPA caused a significant increase in UCP-2 and DA D(1) receptor gene expression in the striatum and both effects were attenuated by pre-treatment with LC. Since LC maintains the ATP/ADP ratio and was previously shown to be neuroprotective against 3-NPA toxicity, the modulation of UCP-2 expression by LC suggests that LC counteracts energy dissipation and thus prevents the negative effects of ATP decline on DA neurotransmission.