Platelet aggregating activity and metastatic remission of human lung adenocarcinoma cell line (KUM·LK-2)

• Published in: Blood & Vessel Vol. 19; no. 3; pp. 280 - 282
• Main Authors: NAKANO, Keiji, YASUTOMI, Masayuki, SAGARA, Noriyuki, AIZAWA, Masuo, INUFUSA, Haruhiko
• Format: Journal Article
• Language: English
• Published: The Japanese Society on Thrombosis and Hemostasis 1988
• Subjects: DN 9693' platelet aggregating material; human lung adenocarcinoma cell line; natual killer activity
• ISSN: 0386-9717; 1884-2372; 1884-2372
• DOI: 10.2491/jjsth1970.19.280

We reported previously the establishment and platelet aggregating activity of human lung adenocarcinoma cell line KUM·LK-2 which produce spontaneous metastasis in nude mouse. Platelet aggregating potential in vivo and vitro, and metastatic potential of KUM·LK-2 were performed under using anti-platelet agent DN 9693 which owned phosphodiesterase blocking activity and not owned anti-cancer activity. DN 9693 blocked platelet aggregating potential and fatal acute thromboembolism of nude mouse induced by KUM·LK-2 cell. KUM·LK-2 metastases were produced subcutaneous implantation group only. But in the case of naturall killer remission using anti-asialo GM1, KUM·LK-2 produce metastases intravenous injection group too. DN 9693 blocked the metastasis of KUM·LK-2 in the case of natural killer reduced nude mouse completely. From these results DN 9693 blocked platelet aggregating activity of KUM·LK-2 cell in vivo and vitro, and blocked metastasis. It was concluded that platelet aggregating activity of KUM·LK-2 cell is necessery to produce metastasis. It was first experiment of human cancer metastasis blocking using anti-platelet agent and proof that anti-platelet agent may usefull for anti-metastasis of human cancer.