Study on Intratumor Heterogenity of Nuclear DNA Content in Colorectal Carcinoma
The DNA content of five samples obtained from different sites in each of 67 colorectal carcinomas was measured by flow cytometry to study on intratumor heterogenity of nuclear DNA content. Among the five samples of the same tumor DNA ploidy was discordant in 16 cases and the difference in the DNA in...
Saved in:
| Published in | Nippon Daicho Komonbyo Gakkai Zasshi Vol. 45; no. 3; pp. 266 - 272 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
The Japan Society of Coloproctology
1992
|
| Online Access | Get full text |
| ISSN | 0047-1801 1882-9619 |
| DOI | 10.3862/jcoloproctology.45.266 |
Cover
| Abstract | The DNA content of five samples obtained from different sites in each of 67 colorectal carcinomas was measured by flow cytometry to study on intratumor heterogenity of nuclear DNA content. Among the five samples of the same tumor DNA ploidy was discordant in 16 cases and the difference in the DNA index (DI) value was found in 11 patients with aneuploidy in all specimens taken from the same tumors. Therefore intratumor DNA heterogenity was observed in twentyseven of 67 cased (40.7 %). DNA heterogenity was not associated with the location of the tumor, size, depth of tumorinvasion, lymphatic vessel invasion, lymph node metastasis, and stage. On the other hand, tumors with the presence of DNA heterogenity had significantly higher frequency of moderately differentiated adenocarcinoma, presence of venous invasion, and liver metastasis than those wdthout DNA heterogenity. The incidence of tumor with s (a2) or si (ai), liver metastasis and advanced stage more than stage III was highest in the cases with aneuploidy in all samples and the presence of intratumor DNA heterogenity. There was a good correlation between DI obtained from biopsy and resected specimen only when intratumor DNA heterogenity was not observed. |
|---|---|
| AbstractList | The DNA content of five samples obtained from different sites in each of 67 colorectal carcinomas was measured by flow cytometry to study on intratumor heterogenity of nuclear DNA content. Among the five samples of the same tumor DNA ploidy was discordant in 16 cases and the difference in the DNA index (DI) value was found in 11 patients with aneuploidy in all specimens taken from the same tumors. Therefore intratumor DNA heterogenity was observed in twentyseven of 67 cased (40.7 %). DNA heterogenity was not associated with the location of the tumor, size, depth of tumorinvasion, lymphatic vessel invasion, lymph node metastasis, and stage. On the other hand, tumors with the presence of DNA heterogenity had significantly higher frequency of moderately differentiated adenocarcinoma, presence of venous invasion, and liver metastasis than those wdthout DNA heterogenity. The incidence of tumor with s (a2) or si (ai), liver metastasis and advanced stage more than stage III was highest in the cases with aneuploidy in all samples and the presence of intratumor DNA heterogenity. There was a good correlation between DI obtained from biopsy and resected specimen only when intratumor DNA heterogenity was not observed. |
| Author | Yamashita, S. Tsujimoto, S. Hasebe, Y. Funabashi, K. Yanagita, K. Andou, K. Nagasawa, Y. Tsujita, K. Kuramoto, S. Kobayashi, K. Kuwabara, T. Watanabe, M. Torikoshi, Y. Yoshio, T. Gotou, T. |
| Author_xml | – sequence: 1 fullname: Funabashi, K. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Yanagita, K. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Kuramoto, S. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Andou, K. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Yoshio, T. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Tsujita, K. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Torikoshi, Y. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Yamashita, S. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Kobayashi, K. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Kuwabara, T. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Nagasawa, Y. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Hasebe, Y. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Tsujimoto, S. organization: Department of Pathology, Toho University Omori Hospital – sequence: 1 fullname: Gotou, T. organization: First Departhent of Surgery, School of Medicine, Toho Universty – sequence: 1 fullname: Watanabe, M. organization: First Departhent of Surgery, School of Medicine, Toho Universty |
| BookMark | eNpdkM9uwjAMxqOJSWOMV5jyAmVxm4ZwROwfEoLDuFdu6rCikqA0HHj7BTFx4OLPsv37JH_PbOC8I8ZeQUwKrfK3vfGdPwZvYtLdeSLLSa7UAxuC1nk2UzAbsKEQcpqBFvDExn3f1kLkUktVlkO2-Ymn5sy940sXA8bTwQf-TZGC35FrY1pZvj6ZjjDw9_WcL7yL5CJvXWo7H8hE7PgCg2mdP-ALe7TY9TT-1xHbfn5sF9_ZavO1XMxXmclzqTIA1AoMQFGWtpFaKKFFYwVYIWslalsbC0Q1WrSkAacyDeqmQColWVuMmLramuD7PpCtjqE9YDhXIKpLMNVdMJUsqxRMAjdXcN9H3NENwxDb9OQ9BjM1vaDFtSSH26X5xVCRK_4A_-F87w |
| ContentType | Journal Article |
| Copyright | The Japan Society of Coloproctology |
| Copyright_xml | – notice: The Japan Society of Coloproctology |
| DBID | AAYXX CITATION |
| DOI | 10.3862/jcoloproctology.45.266 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| EISSN | 1882-9619 |
| EndPage | 272 |
| ExternalDocumentID | 10_3862_jcoloproctology_45_266 article_jcoloproctology1967_45_3_45_3_266_article_char_en |
| GroupedDBID | 123 2WC ABDBF ALMA_UNASSIGNED_HOLDINGS CS3 F5P JSF KQ8 OK1 RJT AAYXX CITATION |
| ID | FETCH-LOGICAL-c2246-11a861c11355fd4806080df01f04b60bfbcf1eebafafe81a74bcfbd3ae54eff3 |
| ISSN | 0047-1801 |
| IngestDate | Tue Jul 01 03:53:56 EDT 2025 Wed Sep 03 06:31:28 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c2246-11a861c11355fd4806080df01f04b60bfbcf1eebafafe81a74bcfbd3ae54eff3 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/jcoloproctology1967/45/3/45_3_266/_article/-char/en |
| PageCount | 7 |
| ParticipantIDs | crossref_primary_10_3862_jcoloproctology_45_266 jstage_primary_article_jcoloproctology1967_45_3_45_3_266_article_char_en |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | 1992 1992-00-00 |
| PublicationDateYYYYMMDD | 1992-01-01 |
| PublicationDate_xml | – year: 1992 text: 1992 |
| PublicationDecade | 1990 |
| PublicationTitle | Nippon Daicho Komonbyo Gakkai Zasshi |
| PublicationTitleAlternate | Nippon Daicho Komonbyo Gakkai Zasshi |
| PublicationYear | 1992 |
| Publisher | The Japan Society of Coloproctology |
| Publisher_xml | – name: The Japan Society of Coloproctology |
| References | 24) Toshima K, Nagorney DM, Rainwater LM, et al : Prognostic significance of nuclear deoxyribonucleic acid ploidy patterns in rese-cted hepatic metastasis from colorectal carcinoma. Surgery 102 : 635-641, 1987 17) Hiddemann W, Von Basewitz DB, Kleinmer H, et al: DNA stemline heterogeneity in colorectal cancer. Cancer 58 : 258-263, 1986 16) Tribukait B, Hammarrberg C, Rubio C : Ploidy and proliferation patterns in colorectal adenocarcinomas related to Dukes' classification and to histopathological differentiation. Acta Pathol Microbiol Immunol Scand Sect A 91 : 89-95, 1983 11) 鈴木宏志,山村剛司,松本好市ほか:大腸癌の腫瘍細胞のDNA ploidyと術後累積生存率の関連.日本大腸肛門病会誌43:1419-1422,1990 12) Rognum TO, Thorud E, Lund E : Survival of large bowel carcinoma patients with different DNA ploidy. Br J Cancer 56 : 633-636, 1987 5) Kokal WA, Garden RL, Sheibani K, et al : Tumor DNA content in resectable primary colorectal carcinoma. Ann Surg 209 : 188-193, 1989 7) 武川啓一:直腸癌とDNA-予後因子としてのDNA量測定の意義-.日外会誌91:980-986,1990 2) 安藤善郎:DNA flowcytometryからみた大腸癌の予後4関する検討.日外会誌911700-1709,1990 4) Scott NA, Rainwater LM, Wieand HS, et al: The relative prognostic value of flow cytometric DNA analysis and conventional clinicopathologic criteria in patients with operable rectal carcinoma. Dis Colon Rectum 30 : 513-520, 1987 22) 丸岡康洋,前谷俊三,戸部隆吉:大腸癌の予後に関する病理学的因子の統計学的解析.日外会誌89:181-191,1988 10) 辻田和紀,船橋公彦,渡邊正志ほか:Fiowcy-tometryによる大腸癌核DNA量測定の意義.日消外会誌24:2176-2182,1991 8) 小坂健夫,伊井徹,松本尚ほか:FCMをもちいたDNA PIoidyおよびDNA Indexと大腸癌の予後に関する検討.日本大腸肛門病会誌43:61-66,1990 19) Scott NA, Grande JP, Weiland LH, et al : Flow cytometric DNA patterns from colorectal cancers. -How reproducible are they? Mayo Clin Proc 72: 331-337, 1987 9) Giaretti W, Danova M, GTido E, et al: Flow cytometric DNA index in the prognosis of colorectal cancer. Cancer 67 : 1921- 1927, 1991 3) 石川啓,田川泰,中越享ほか:多変量解析による大腸癌核DNA量の予後因子としての評価.日消外会誌22:1806-1860,1989 21) 飯田明:大腸癌の組織型と治療成積.とくに高分化腺癌と中分化腺癌の生存率の違いについて,目本大腸肛門病会誌43:533-541,1990 20) Wersto RP, Liblit RL, Deitch D, et al: Variability in DNA measurements in multiple tumor samples of human colonic carcinoma. Cancer 67 : 106-115, 1991 23) 山口明夫,石田哲也,西村元一ほか:核DNAよりみた大腸癌転移例(FCMを用いた検討).日外会誌91:1591-1595,1990 15) Merkel ED, Mcguire LW : Ploidy, proliferative activity and prognosis DNA flow cytometry of solid tumors. Cancer 65 : 1194- 1205, 1990 1) Wolley RC, Schreiber K, Koss LG, et al: DNA distribution in human colon carcino-mas and its relationship to clinical behavior. J Natl Cancer Inst 69 : 15-22, 1982 6) Jones DJ, Moore M, Schofield PF : Prognostic significance of DNA ploidy in colorectal cancer : A prospective flow cytometric study. Br J Surg 75 : 28-33, 1988 18) Sasaki K, Hashimoto T, Kawachino K, et al: Intratumoral regional differences in DNA ploidy of gastrointestinal carcinomas. Cancer 62 : 2569-2575, 1988 13) Heppner GH : Tumor heterogeneity. Cancer Res 44, 2259-2265, 1984 14) 大腸癌研究会編:大腸癌取扱い規約.改訂第4版.東京,金原出版,1985 |
| References_xml | – reference: 10) 辻田和紀,船橋公彦,渡邊正志ほか:Fiowcy-tometryによる大腸癌核DNA量測定の意義.日消外会誌24:2176-2182,1991 – reference: 14) 大腸癌研究会編:大腸癌取扱い規約.改訂第4版.東京,金原出版,1985 – reference: 23) 山口明夫,石田哲也,西村元一ほか:核DNAよりみた大腸癌転移例(FCMを用いた検討).日外会誌91:1591-1595,1990 – reference: 11) 鈴木宏志,山村剛司,松本好市ほか:大腸癌の腫瘍細胞のDNA ploidyと術後累積生存率の関連.日本大腸肛門病会誌43:1419-1422,1990 – reference: 15) Merkel ED, Mcguire LW : Ploidy, proliferative activity and prognosis DNA flow cytometry of solid tumors. Cancer 65 : 1194- 1205, 1990 – reference: 1) Wolley RC, Schreiber K, Koss LG, et al: DNA distribution in human colon carcino-mas and its relationship to clinical behavior. J Natl Cancer Inst 69 : 15-22, 1982 – reference: 17) Hiddemann W, Von Basewitz DB, Kleinmer H, et al: DNA stemline heterogeneity in colorectal cancer. Cancer 58 : 258-263, 1986 – reference: 20) Wersto RP, Liblit RL, Deitch D, et al: Variability in DNA measurements in multiple tumor samples of human colonic carcinoma. Cancer 67 : 106-115, 1991 – reference: 6) Jones DJ, Moore M, Schofield PF : Prognostic significance of DNA ploidy in colorectal cancer : A prospective flow cytometric study. Br J Surg 75 : 28-33, 1988 – reference: 3) 石川啓,田川泰,中越享ほか:多変量解析による大腸癌核DNA量の予後因子としての評価.日消外会誌22:1806-1860,1989 – reference: 16) Tribukait B, Hammarrberg C, Rubio C : Ploidy and proliferation patterns in colorectal adenocarcinomas related to Dukes' classification and to histopathological differentiation. Acta Pathol Microbiol Immunol Scand Sect A 91 : 89-95, 1983 – reference: 19) Scott NA, Grande JP, Weiland LH, et al : Flow cytometric DNA patterns from colorectal cancers. -How reproducible are they? Mayo Clin Proc 72: 331-337, 1987 – reference: 21) 飯田明:大腸癌の組織型と治療成積.とくに高分化腺癌と中分化腺癌の生存率の違いについて,目本大腸肛門病会誌43:533-541,1990 – reference: 9) Giaretti W, Danova M, GTido E, et al: Flow cytometric DNA index in the prognosis of colorectal cancer. Cancer 67 : 1921- 1927, 1991 – reference: 4) Scott NA, Rainwater LM, Wieand HS, et al: The relative prognostic value of flow cytometric DNA analysis and conventional clinicopathologic criteria in patients with operable rectal carcinoma. Dis Colon Rectum 30 : 513-520, 1987 – reference: 8) 小坂健夫,伊井徹,松本尚ほか:FCMをもちいたDNA PIoidyおよびDNA Indexと大腸癌の予後に関する検討.日本大腸肛門病会誌43:61-66,1990 – reference: 5) Kokal WA, Garden RL, Sheibani K, et al : Tumor DNA content in resectable primary colorectal carcinoma. Ann Surg 209 : 188-193, 1989 – reference: 24) Toshima K, Nagorney DM, Rainwater LM, et al : Prognostic significance of nuclear deoxyribonucleic acid ploidy patterns in rese-cted hepatic metastasis from colorectal carcinoma. Surgery 102 : 635-641, 1987 – reference: 13) Heppner GH : Tumor heterogeneity. Cancer Res 44, 2259-2265, 1984 – reference: 18) Sasaki K, Hashimoto T, Kawachino K, et al: Intratumoral regional differences in DNA ploidy of gastrointestinal carcinomas. Cancer 62 : 2569-2575, 1988 – reference: 7) 武川啓一:直腸癌とDNA-予後因子としてのDNA量測定の意義-.日外会誌91:980-986,1990 – reference: 22) 丸岡康洋,前谷俊三,戸部隆吉:大腸癌の予後に関する病理学的因子の統計学的解析.日外会誌89:181-191,1988 – reference: 12) Rognum TO, Thorud E, Lund E : Survival of large bowel carcinoma patients with different DNA ploidy. Br J Cancer 56 : 633-636, 1987 – reference: 2) 安藤善郎:DNA flowcytometryからみた大腸癌の予後4関する検討.日外会誌911700-1709,1990 |
| SSID | ssib002484655 ssib002671002 ssj0064967 |
| Score | 1.3111843 |
| Snippet | The DNA content of five samples obtained from different sites in each of 67 colorectal carcinomas was measured by flow cytometry to study on intratumor... |
| SourceID | crossref jstage |
| SourceType | Index Database Publisher |
| StartPage | 266 |
| Title | Study on Intratumor Heterogenity of Nuclear DNA Content in Colorectal Carcinoma |
| URI | https://www.jstage.jst.go.jp/article/jcoloproctology1967/45/3/45_3_266/_article/-char/en |
| Volume | 45 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Nippon Daicho Komonbyo Gakkai Zasshi, 1992, Vol.45(3), pp.266-272 |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 1882-9619 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0064967 issn: 0047-1801 databaseCode: KQ8 dateStart: 19670101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELaWwoELAgGi5SEfuK2yjTeOkxwr2rKiYhHqIlVcIj9ikVZNVqvkAL-Mn8dM7E2yqx6g4hKtLNtyZr6dV2bGhLyHv79iIjRBolIVcKFUkJowDgzXMpGZzEKN8Y7PS7H4xj9dxVeTye9R1lLbqJn-dWddyX24CmPAV6yS_QfO9pvCAPwG_sITOAzPv-LxpesI3cX1sDfybb0BPQKUqmGJz7VYYsNiuZmeLrEAHRPWG1fpB446CDuMHeB1QlXtBbQ3VJfleg0bn8oS5OP0ooZ3Uj_r6Ud5cyPL6XcwuX-UPe_bSiq8lKmTGrNekHRXIDnrdBi9aDcS4NFFaC_70ZPK1O1oovGFeYO_2tWwgWav-kRTTCSBt0AV3AwfB7wE5knAUh_AKJzQRSs_E150eqnsmkx69EVjESvESFvP3cU_-4ogAkcNFYHeOcaMx7N--U6Tbc_CfG8BiKck53EeuQeszbczsSgOMPiAPJwnYONgOsDXsSOX7jamE9hFqW9sJngmXDdXTw1Xu46nPr77zDtm06Nr8By2WYedIbR6Sp54D4aeuBM-I5Oiek6-dFCkdUUHKNIxFGltqYciBShSD0VaVnSAIu2h-IKszs9WHxaBv6sj0NiSMGBMpoJpxsB-tYanoQBXxNiQ2ZArESqrtGVFoaSVtkiZTDgMKBPJIuaFtdFLclDVVfGKUNA5MFWHNlMMrPtIGW7TOTNWxzpidn5IjreUyNeuI0sOnizSbp99yDWg3SFZOIL18-_N7qP_t9Vr8tilfmM47w05aDZt8RYM3Ea967D0B9fEsyo |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Study+on+Intratumor+Heterogenity+of+Nuclear+DNA+Content+in+Colorectal+Carcinoma&rft.jtitle=Nippon+Daicho+Komonbyo+Gakkai+Zasshi&rft.au=Funabashi%2C+K.&rft.au=Yanagita%2C+K.&rft.au=Kuramoto%2C+S.&rft.au=Andou%2C+K.&rft.date=1992&rft.pub=The+Japan+Society+of+Coloproctology&rft.issn=0047-1801&rft.eissn=1882-9619&rft.volume=45&rft.issue=3&rft.spage=266&rft.epage=272&rft_id=info:doi/10.3862%2Fjcoloproctology.45.266&rft.externalDocID=article_jcoloproctology1967_45_3_45_3_266_article_char_en |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0047-1801&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0047-1801&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0047-1801&client=summon |