Study on Intratumor Heterogenity of Nuclear DNA Content in Colorectal Carcinoma

• Published in: Nippon Daicho Komonbyo Gakkai Zasshi Vol. 45; no. 3; pp. 266 - 272
• Main Authors: Funabashi, K., Yanagita, K., Kuramoto, S., Andou, K., Yoshio, T., Tsujita, K., Torikoshi, Y., Yamashita, S., Kobayashi, K., Kuwabara, T., Nagasawa, Y., Hasebe, Y., Tsujimoto, S., Gotou, T., Watanabe, M.
• Format: Journal Article
• Language: English
• Published: The Japan Society of Coloproctology 1992
• ISSN: 0047-1801; 1882-9619
• DOI: 10.3862/jcoloproctology.45.266

The DNA content of five samples obtained from different sites in each of 67 colorectal carcinomas was measured by flow cytometry to study on intratumor heterogenity of nuclear DNA content. Among the five samples of the same tumor DNA ploidy was discordant in 16 cases and the difference in the DNA index (DI) value was found in 11 patients with aneuploidy in all specimens taken from the same tumors. Therefore intratumor DNA heterogenity was observed in twentyseven of 67 cased (40.7 %). DNA heterogenity was not associated with the location of the tumor, size, depth of tumorinvasion, lymphatic vessel invasion, lymph node metastasis, and stage. On the other hand, tumors with the presence of DNA heterogenity had significantly higher frequency of moderately differentiated adenocarcinoma, presence of venous invasion, and liver metastasis than those wdthout DNA heterogenity. The incidence of tumor with s (a2) or si (ai), liver metastasis and advanced stage more than stage III was highest in the cases with aneuploidy in all samples and the presence of intratumor DNA heterogenity. There was a good correlation between DI obtained from biopsy and resected specimen only when intratumor DNA heterogenity was not observed.