Expression Changes of Early Response Genes in Lung Due to High Volume Ventilation

• Published in: Current medical science Vol. 25; no. 3; pp. 339 - 342
• Main Author: 王月兰 姚尚龙 熊平
• Format: Journal Article
• Language: English
• Published: China Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2005
• Subjects: Animals; Gene Expression Profiling; Genes, Immediate-Early; Immediate-Early Proteins - biosynthesis; Immediate-Early Proteins - genetics; Lung - metabolism; Proto-Oncogene Proteins c-jun - biosynthesis; Proto-Oncogene Proteins c-jun - genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Respiration, Artificial - adverse effects; Ventilators, Mechanical - adverse effects; 基因表达; 潮汐量; 肺疾病; 通风技术; early response gene; transcription factor; mechanical ventilation; lung injury
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/bf02828161

The expression changes of early response genes due to ventilation with high volume in adult rats in vivo were observed. Forty SD male rats were randomly divided into control and 30, 60，90 and 120min ventilation groups, respectively (n=8 in each group). The animals were ventilated with tidal volume of 42 ml/kg and a PEEP level of 0 cmH2O at a rate of 40 breaths per minute in room air with a ventilator was given to the small animals. The expression of Egr-1, C-jun and IL-1β mRNA and proteins was detected by RT-PCR and immunohistochemical technique, respectively. The pathological changes in lung tissues were examined by HE staining. The results indicated that the expression of Egr-1, C-jun and IL-1β mRNA was detectable at 30th min after overventilation, but there was no significant difference in comparison with that in control group until overventilation for 60 min. However, at 90 and 120 min there was a significent increase as compared with 30 min or control group (P<0.05). The expression of Egr-1, C-jun and IL-1β deteced by immunohistochemical assay also showed a similar tendency of the gradual increase. In the 120 min ventilation group, the expression intensity of Egr-1, C-jun and IL-1β proteins in lung cells was the strongest and the nuclear translocation was increased markedly in comparison with any other groups (P<0.05). HE staining suggested that the degree of lung injury was aggravated gradually with the ventialtion going on and had a similar tendency to the expression of these early response genes """and proteins. The current data suggested that overventilation activated and upregulated the expression of early response genes and the expression of these genes may be taken as the early signal to predict the onset and degree of lung injury. These results may demonstrated partially that the expression of early response genes induced by the mechanical stretch is associated with biochamic lung injury.