THE EXPRESSION OF APOPTOSIS RELATED GENES IN THE PROCESS OF CANCERATION OF ATYPICAL HYPERPLASIA OF MAMMARY DUCT

• Published in: Chinese journal of cancer research Vol. 18; no. 1; pp. 45 - 50
• Main Author: 李柏林 马萍 宋敏 宋继谒
• Format: Journal Article
• Language: English
• Published: Department of Chemistry, College of Basic Medical Sciences, China Medical University, Shenyang 110001%Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang 110001 01.03.2006
• Subjects: 乳腺导管; 基因表达; 细胞凋亡; Expression of gene; Apoptosis; Atypical hyperplasia of mammary duct
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/s11670-006-0045-6

Objective： To investigate the expression of apoptosis related genes p53 and bcl-2 in atypical hyperplasia of mammary duct and the relationship between the gene expression and oncogenesis of breast. Methods： mRNA of apoptosis related gene p53 and bcl-2 were detected by in situ hybridization in 44 cases of atypical ductal hyperplasia. p53 protein expression was detected by immunohistochemistry. The data were compared with those of 6 cases of benign hyperplasia and 26 cases of breast carcinoma. Results： The expression of p53 mRNA was 66.7% in benign hyperplasia, 40% in atypical ductal hyperplasia （55.6% in mild, 41.7% in medium, 26.1% in severe） and 19.2% in carcinoma （of which 21.4% were intraductal carcinoma and 16.7% were invasive）. The expression of p53 protein was negative in benign hyperplasia, 24% in atypical hyperplasia （mild 11.1%, medium 25%, severe 34.8%）, 38.5% in carcinoma （intraductal carcinoma 35.7%, invasive ductal carcinoma 41.7%）. The expression of bcl-2 was negative in benign hyperplasia, 78.6% in intraductal carcinoma, 83.3% in invasive ductal carcinoma. Conclusion： Loss and mutation of p53 gene and excessive expression bcl-2 mRNA were detected in severe atypical ductal hyperplasia.