DETECTION OF SBEM-MRNA IN PERIPHERAL BLOOD OF PATIENTS WITH BREAST CANCER AND ITS CLINICAL SIGNIFICANCE

• Published in: Chinese journal of cancer research Vol. 18; no. 4; pp. 294 - 298
• Main Author: 杨华伟 杨南武 曹骥 刘剑仑 张传珉 陈建思 蒋奕 欧超 苏建家
• Format: Journal Article
• Language: English
• Published: Department of Gastroenteritis &Breast%Department of Pathology, Affiliated Cancer Hospital, Guangxi Medical University, Nanning, 530021 01.12.2006
• Subjects: 乳腺肿瘤; 化学治疗; 外围血管; 病理特征; breast cancer; Small breast epithelial mucin mRNA; Nested-RT-PCR; Peripheral blood
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/s11670-006-0294-4

Objective： The aim of the present study is to explore the expression of a specific marker of breast cancer, small breast epithefial mucin （SBEM） mRNA, in peripheral blood and to investigate its significance. Methods： The expressions of SBEM-mRNA in peripheral blood of 67 patients with breast cancer, 16 patients with benign breast disease, and 20 normal healthy volunteers were detected with nested reverse transcription-polymerase chain reaction （Nested-RT-PCR）. Results： SBEMomRNA was negative in healthy individuals and patients with benign breast tumor, but 50.7%（34/67） of breast cancer patients showed positive expression of SBEM-mRNA in peripheral blood, of whom 25%（2/8） were in stage Ⅰ, 45.8%（11/24） in stage Ⅱ, 43.75%（11/24） in stage Ⅲ and 73.7（14/19） in stage Ⅳ. The positive rate in stage Ⅳ was higher than that in stage Ⅰ, Ⅱ, Ⅲ （P〈0.05）. Expressions of SBEM-mRNA had no correlation with age, carcinoma size, pathological type, ER and PR state （P〉0.05）. Concision： SBEM-mRNA is specifically expressed in breast cancer and it may act as a marker for the detection of of breast cancer.