ANALYTICAL METHOD OPTIMIZATION AND VALIDATION OF GLIBENCLAMIDE AND METFORMIN HYDROCHLORIDE IN DIABETIC HERBS PRODUCT BY THIN-LAYER CHROMATOGRAPHY-DENSITOMETRY

• Published in: International journal of applied pharmaceutics pp. 83 - 87
• Main Authors: MAGGADANI, BAITHA PALANGGATAN, SUGIANTO, NOVIANI, HAYUN
• Format: Journal Article
• Language: English
• Published: 21.03.2020
• ISSN: 0975-7058; 0975-7058
• DOI: 10.22159/ijap.2020.v12s1.FF014

Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting thepresence of glibenclamide and metformin hydrochloride in herbal diabetic products.Methods: The jamu samples were extracted using methanol, followed by analysis using TLC-densitometry with methanol-aquades-glacial acetic acid(9:1:0.25) as the mobile phase at a wavelength of 237 nm.Results: From the validation results, the correlation coefficients for glibenclamide and metformin hydrochloride were determined to be 0.9998and 0.9981, respectively. The limits of detection and quantitation for glibenclamide were 49.97 μg/mL and 66.55 μg/mL, respectively, while thoseof metformin hydrochloride were 74.75 μg/mL and 249.25 μg/mL, respectively. The method developed in this study met the criteria of selectivity,accuracy, and precision. From seven jamu samples tested, four tested positive for glibenclamide, with sample 1=4.9522%, sample 2=4.1495%, sample3=4.2578%, and sample 4=4.9412%.Conclusion: None of the jamu samples contained metformin hydrochloride.