Effects of salvianolic acids on endothelial cells against damage induced by cholestane-3β-5α-6β-triol

Objective To investigate the effects of salvianolic acids on human umbilical vein endothelial cells(HUVEC) against damage induced by cholestane-3β-5α-6β-triol (chol-triol).Methods The viability of HUVEC was measured by MTT method. The apoptosis of HUVEC induced by chol-triol was detected by flow cyt...

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Bibliographic Details
Published inChinese medical journal Vol. 116; no. 4; pp. 630 - 632
Main Author 任德成 杜冠华 张均田
Format Journal Article
LanguageEnglish
Published Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China 01.04.2003
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ISSN0366-6999
2542-5641

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Summary:Objective To investigate the effects of salvianolic acids on human umbilical vein endothelial cells(HUVEC) against damage induced by cholestane-3β-5α-6β-triol (chol-triol).Methods The viability of HUVEC was measured by MTT method. The apoptosis of HUVEC induced by chol-triol was detected by flow cytometry and TUNEL assay. The production of malondialdehyd (MDA) in HUVEC was tested by thiobarbaturic acid (TBA) assay.Results The viability of HUVEC treated with chol-triol 100μmol/L decreased by 39. 8% while salvianolic acids 100μg/ml increased by 27. 9%. The apoptotic rate of HUVEC measured by PI staining increased from 6% -8% to 17% -20% after chol-triol treatment for 12 h. Salvianolic acids100μg/ml reduced the apoptotic rate to 10% -14% after treatment HUVEC for 1 h prior to chol-triol treatment. In another experiment, chol-triol increased the number of TUNEK-positive cells 5 times, but salvianolic acids 10μg/ml and 100μg/ml reduced the number of TUNEL-positive cells by 36. 9% and 61.2%, respectively. The production of MDA in HUVEC increased by 120. 7% after chol-triol treatment for 12 h. Salvianolic acids 10 gg/ml and 100 pg/ml also decreased the concentration of MDA by 28.7% and 39. 8%, respectively.Conclusion Salvianolic acids has protective effect on endothelial cells against damage induced by chol-triol.
Bibliography:11-2154/R
R329.2
ISSN:0366-6999
2542-5641