Hyaluronic acid as a biomaterial for dental pulp regeneration: a systematic review of preclinical studies

• Published in: Frontiers in bioengineering and biotechnology Vol. 13; p. 1661188
• Main Authors: Al-Masoody, Azal Hadi, Hodjat, Mahshid, Kashi, Tahereh Sadat Jafarzadeh
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2025
• Subjects: dental pulp; dentinogenesis; endodontic regeneration; hyaluronic acid; vital pulp therapy (VPT); dental pulp; dentinogenesis; endodontic regeneration; vital pulp therapy (VPT); hyaluronic acid
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2025.1661188

With the current advancements in regenerative medicine, it has become necessary to refine the current regenerative endodontic procedure (REP). Scaffold improvement, including the use of hyaluronic acid as a natural biomaterial, has been the subject of many studies. This systematic review aims to explore the effects of hyaluronic acid (HA) on dental pulp regeneration. A comprehensive search on Hinari, PubMed, Springer, and ScienceDirect databases in July 2024 was carried out. All and animal studies that assessed the effects of HA on cell vitality, proliferation, dentinogenesis, neovascularisation and neurogenesis in an endodontic context were included. Clinical studies were excluded. All articles were screened and assessed for relevance by the authors. The quality and risk of bias of the included studies were evaluated using the QUIN, SYRCLE, and ARRIVE Essential 10 tools. A total of 23 articles were included, comprising 17 , 5 animal, and 1 combined and animal study. Thematic synthesis of results was adopted. The methodologies for HA addition, HA concentration and molecular weight were different across the articles. Most studies showed that HA have a neutral effect on cell proliferation, and a positive effect on dentinogenesis and neovascularisation. Most animal studies showed increased dentine bridge formation. The variability in the study design has made it difficult to assert the results; however, most studies agree that HA has promising potential in REP. [https://osf.io/t45ec/].