α 1-Antitrypsin and serum albumin mRNA accumulation in normal, acute phase and ZZ human liver

• Published in: FEBS letters Vol. 189; no. 2; pp. 361 - 366
• Main Authors: Riley, J.H., Bathurst, I.C., Edbrooke, M.R., Carrell, R.W., Craig, R.K.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23.09.1985
• Subjects: Acute phase protein; Adolescent; Adult; alpha 1-Antitrypsin - genetics; Amino Acid Sequence; Base Sequence; C-Reactive Protein - analysis; cDNA sequence α 1-antitrypsin; DNA - metabolism; Female; Humans; Liver - metabolism; Liver mRNA; Male; Middle Aged; RNA, Messenger - metabolism; Serum Albumin - genetics; Time Factors; Z human α 1-antitrypsin; Z human α 1-antitrypsin; Acute phase protein; Liver mRNA; cDNA sequence α 1-antitrypsin
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(85)81056-5

α 1-Antitrypsin and albumin mRNA levels of 4 human livers were assessed using a newly sequenced cDNA clone of the carboxyterminal third of α 1-antitrypsin and a previously cloned albumin cDNA sequence. The relative concentration of α 1-antitrypsin mRNA was the same in poly(A)-containing RNA isolated from acute phase (MM) and α 1-antitrypsin deficient (ZZ) individuals. In the acute phase liver relative to the normal (MM) liver, total RNA extracts showed a marked decrease in albumin mRNA concentration but no increase in α 1-antitrypsin mRNA. The ZZ liver showed decreased total and poly(A)-containing RNA content but the same proportion of α-antitrypsin to albumin mRNA as in the normal (MM) liver. This supports other evidence that ZZ α 1-antitrypsin deficiency is due to a defect in polypeptide processing (secretion) rather than a deficiency in mRNA accumulation.