Inhibition of leukotriene B4 formation in human polymorphonuclear leukocytes by halothane

The effect of halothane (2-bromo-2-chloro-1, 1, 1-trifluoroethane), an inhalation anesthetic, on the formation of leukotriene B4 (LTB4) was studied in human polymorphonuclear leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of LTB4 in a dose-dependent manner and...

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Published inEnsho Vol. 6; no. 4; pp. 397 - 399
Main Authors Minakami, Shigeki, Takeshige, Koichiro, Yoshitake, Junichi, Sumimoto, Hideki, Yamaoka, Atsushi
Format Journal Article
LanguageJapanese
Published The Japanese Society of Inflammation and Regeneration 1986
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ISSN0389-4290
1884-4006
1884-4006
DOI10.2492/jsir1981.6.397

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Abstract The effect of halothane (2-bromo-2-chloro-1, 1, 1-trifluoroethane), an inhalation anesthetic, on the formation of leukotriene B4 (LTB4) was studied in human polymorphonuclear leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of LTB4 in a dose-dependent manner and the inhibition was restored by the removal of halothane.
AbstractList The effect of halothane (2-bromo-2-chloro-1, 1, 1-trifluoroethane), an inhalation anesthetic, on the formation of leukotriene B4 (LTB4) was studied in human polymorphonuclear leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of LTB4 in a dose-dependent manner and the inhibition was restored by the removal of halothane.
Author Yamaoka, Atsushi
Sumimoto, Hideki
Takeshige, Koichiro
Minakami, Shigeki
Yoshitake, Junichi
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  fullname: Sumimoto, Hideki
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References 4) Sumimoto, H., Takeshige, K., Minakami, S.: Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4. Biochim. Biophys. Acta 803: 271-277, 1984.
7) Bruce, D.L.: Effect of halothane anesthesia on experimental Salmonella peritonitis in mice. J. Surg. Res. 7: 180-185, 1967.
12) Abe, M., Kawazoe, Y., Shigematsu, N.: Influences of salts on high-performance liquid chromatography of leukotrienes. Anal. Biochem. 144: 417-422, 1985.
13) Vigo, C., Lewis, G.P., Piper, P.J.: Mechanisms of inhibition of phospholipase A2. Biochem. Pharmacol.: 623-627, 1980.
2) Goetzl, E.J., Pickett, W.C.: The human PMN leukocyte chemotactic activity of complex hydroxy-eicosatetraenoic acids (HETEs) . J. Immunol. 125: 1789-1791, 1980.
5) Samuelusson, B.: Leukotriens: Mediators of immediate hypersensitivity reaction and inflammation. Science 220: 568-575, 1983.
6) Hill, G.E., Stanley, T.H., Lunn, J.K., Liu, W-S., English, J.B., Loesen, E.A., Kawamura, R., Sentken, C.R., Hill, H.R.: Neutrophil chemotaxis during halothane and halothane-N20 anesthesia in man. Anesth. Analg. 56: 696-702, 1977.
9) Nakagawara, M., Takeshige, K., Takamatsu, J., Takahashi, S., Yoshitake, J., Minakami, S.: Inhibition of superoxide production and Ca2+ mobilization in human neutrophils by halothane, enflurane and isoflurane. Anesthesiology (in press) .
3) Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., Shipoey, M.E., Smith, M. J.H.: Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature 286: 264-265, 1980.
8) Welch, W.D.: Halothane reversibly inhibits human neutrophil bacterial killing. Anesthesiology 55: 650-654, 1981.
11) Powell, W.S.: Rapid extraction of arachidonic acid metabolites from biological samples using octadecylsilyl silica. Methods in Enzymology 86: 467-476, 1982.
10) Böyum, A.: Isolation of mononuclear cells by centrifugation and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand. J. Gun. Lab. Invest. 21 (Suppl. 97) : 77-98, 1968.
14) Schwartz, S.H.: Treatment of status athmaticus with halothane. JAMA 251: 2688-2689, 1984.
1) Borgeat, P., Samuelsson, B.: Arachidonic acid metabolism in polymorphonuclear leukocytes: Effect of ionophore A23187. Proc. Natl. Acad. Sci. USA 76: 2148-2152, 1979.
References_xml – reference: 13) Vigo, C., Lewis, G.P., Piper, P.J.: Mechanisms of inhibition of phospholipase A2. Biochem. Pharmacol.: 623-627, 1980.
– reference: 14) Schwartz, S.H.: Treatment of status athmaticus with halothane. JAMA 251: 2688-2689, 1984.
– reference: 2) Goetzl, E.J., Pickett, W.C.: The human PMN leukocyte chemotactic activity of complex hydroxy-eicosatetraenoic acids (HETEs) . J. Immunol. 125: 1789-1791, 1980.
– reference: 10) Böyum, A.: Isolation of mononuclear cells by centrifugation and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand. J. Gun. Lab. Invest. 21 (Suppl. 97) : 77-98, 1968.
– reference: 11) Powell, W.S.: Rapid extraction of arachidonic acid metabolites from biological samples using octadecylsilyl silica. Methods in Enzymology 86: 467-476, 1982.
– reference: 5) Samuelusson, B.: Leukotriens: Mediators of immediate hypersensitivity reaction and inflammation. Science 220: 568-575, 1983.
– reference: 4) Sumimoto, H., Takeshige, K., Minakami, S.: Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4. Biochim. Biophys. Acta 803: 271-277, 1984.
– reference: 9) Nakagawara, M., Takeshige, K., Takamatsu, J., Takahashi, S., Yoshitake, J., Minakami, S.: Inhibition of superoxide production and Ca2+ mobilization in human neutrophils by halothane, enflurane and isoflurane. Anesthesiology (in press) .
– reference: 7) Bruce, D.L.: Effect of halothane anesthesia on experimental Salmonella peritonitis in mice. J. Surg. Res. 7: 180-185, 1967.
– reference: 12) Abe, M., Kawazoe, Y., Shigematsu, N.: Influences of salts on high-performance liquid chromatography of leukotrienes. Anal. Biochem. 144: 417-422, 1985.
– reference: 6) Hill, G.E., Stanley, T.H., Lunn, J.K., Liu, W-S., English, J.B., Loesen, E.A., Kawamura, R., Sentken, C.R., Hill, H.R.: Neutrophil chemotaxis during halothane and halothane-N20 anesthesia in man. Anesth. Analg. 56: 696-702, 1977.
– reference: 8) Welch, W.D.: Halothane reversibly inhibits human neutrophil bacterial killing. Anesthesiology 55: 650-654, 1981.
– reference: 1) Borgeat, P., Samuelsson, B.: Arachidonic acid metabolism in polymorphonuclear leukocytes: Effect of ionophore A23187. Proc. Natl. Acad. Sci. USA 76: 2148-2152, 1979.
– reference: 3) Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., Shipoey, M.E., Smith, M. J.H.: Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature 286: 264-265, 1980.
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SubjectTerms halothane
leukotriene B4
polymorphonuclear leukocyte
Title Inhibition of leukotriene B4 formation in human polymorphonuclear leukocytes by halothane
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