Crude Polysaccharides from Cystoseira species: Extraction, Characterization and in vitro Antioxidant Activity

• Published in: Natural product sciences Vol. 31; no. 2; pp. 102 - 110
• Main Authors: Sabir, Ibtissam, Bouftini, Khadija, Hmidouche, Oumaima, Rchid, Halima, Ursu, Alina-Violeta, Lasky, Mariama, Gardarin, Christine, Moznine, Reddad El, Vial, Christophe, Nmila, Rachid
• Format: Journal Article
• Language: English
• Published: 한국생약학회 30.06.2025
• Subjects: 약학
• ISSN: 1226-3907; 2288-9027
• DOI: 10.20307/nps.2025.31.2.102

Polysaccharides extracted from marine algae have shown important biological activities such as antioxidant properties. In this study, we aim to evaluate the antioxidant properties of natural polysaccharides extracted from three Cystoseira sp. collected from Sidi Bouzid, El Jadida coast, Morocco, using five assays. Polysaccharides were extracted using aqueous methods, then characterized. The hot water extraction yields are 13.5, 12.37, and 11.61% for Cystoseira baccata (CPB), Cystoseira myriophylloides (CPMy) and Cystoseira mediterranea (CPM), respectively. Colorimetric assay analysis revealed that the extracts showed high percentages of total sugars (37 to 54%) and sulfate (5.97 to 6.86%) and low content of phenolic compounds (0.5 to 4%) and protein (5 to 8%). The evaluation of antioxidant properties showed that the crude polysaccharides extracts had significant antioxidant activity in the presence of different radicals. At 5 mg/mL, they exhibited effective DPPH radical-scavenging activity (100% of inhibition for CPM and CPMy and 69.44% for CPB), ABTS radical-scavenging activity (76.14, 71.44 and 100% inhibition for CPB, CPM and CPMy, respectively), Ferric reducing power (110, 137, and 182 µg EAA for CPMy, CPM and CPB respectively), total antioxidant capacity (114, 154, and 323 µg EAA for CPB, CPM and CPMy, respectively), and hydroxyl-radical scavenging activity (68.17, 91.37 and 98.27% for CPB, CPM and CPMy, respectively). Therefore, Cystoseira polysaccharides might be potential antioxidant resources that can be used as a natural compound in pharmaceutical or in the food industry. KCI Citation Count: 0