The Genes Associated with Drought Tolerance by Multi-Layer Approach in Potato

We have developed a multi-layer pipeline that uses transcriptomic data to identify candidate genes associated withcomplex pathways in plants. We used this multi-layer approach, incorporating microarray, RNA sequencing, polymerase chain reaction(PCR), and protein-protein interaction analyses, to iden...

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Published inPlant breeding and biotechnology Vol. 7; no. 4; pp. 405 - 414
Main Authors Kim, Chang-Kug, Oh, Jae-Hyeon, Cho, Chuloh, Kim, Kyung-Hwa, Yu, Go Eun, Kim, Dool-Yi
Format Journal Article
LanguageEnglish
Published 한국육종학회 01.12.2019
Subjects
농학
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ISSN2287-9358
2287-9366
DOI10.9787/PBB.2019.7.4.405

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Summary:We have developed a multi-layer pipeline that uses transcriptomic data to identify candidate genes associated withcomplex pathways in plants. We used this multi-layer approach, incorporating microarray, RNA sequencing, polymerase chain reaction(PCR), and protein-protein interaction analyses, to identify 14 genes associated with drought tolerance in potato. We generatedtransgenic potato lines that over-express bZIP28, one of the genes selected by our pipeline, to confirm the involvement of that gene indrought tolerance. The protein interactions of the products of the screened genes were assessed using yeast two-hybrid and bimolecularfluorescence complementation analyses. Overall, this study demonstrated the utility of our multi-layer approach for decipheringcomplex biosynthetic pathways using transcriptomic data. KCI Citation Count: 0
Bibliography:https://doi.org/10.9787/PBB.2019.7.4.405
ISSN:2287-9358
2287-9366
DOI:10.9787/PBB.2019.7.4.405