Experimental Study of Mouse Cytomegalovirus Infected Mice

• Published in: Current medical science Vol. 22; no. 3; pp. 260 - 264
• Main Author: 崔雯 董永绥 等
• Format: Journal Article
• Language: English
• Published: China Department of Pediatrics, Tongji Hospital, Tongji Medical University,Huazhong University of Science and Technology, Wuhan 430030 2002
• Subjects: Animals; Blood-Brain Barrier; Brain - pathology; Brain - virology; Disease Models, Animal; Female; Herpesviridae Infections - pathology; Herpesviridae Infections - virology; Immunocompromised Host; Liver - pathology; Liver - virology; Lung - pathology; Lung - virology; Mice; Mice, Inbred BALB C; Muromegalovirus; 实验研究; 巨细胞病毒感染; infection; mouse cytomegalovirus
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/BF02828198

In order to investigate the human cytomegalovirus (HCMV) infection,the mouse cytomegalovirus(MCMV) infected mice were experimentally studied 6 to 8 week old female BALB/C mice with immunosuppression were selected to undergo the MCMV inoculations:intracranial inoculation and peritoneal inoculation. MCMV of the infected mice in various organs and tissues were detected by usingβ-gal staining and in situ nucleic acid hybridization assay.The pathological changes were observed in HE staining and in situ nucleic acid hybridization assay.The pathological changes were observed in HE staining paraffin-embedded sections.It was found that all the MCMV infected mice showed the retardation of growth and development,and feather looseness.Both intracranial inoculation of 10^4 PFU viruses or peritoneal inoculation of 10^6PFU viruses resultedin the pathological changes,to some extent,of various organs and tissues in the mice,The pathological changes in liver were consistent with the amount ofβ-gal staining positive the viruses in the immunosuppressed mice subjected to intracranial inoculation could spread to whole body organs,while the viruses in the immunosuppressed mice subjected to intrapeitoneal inoculation couldn't spread to the brain,suggesting blood-brain barrier could prevent the virus from spreading to the brain.