Effects of Triptolide on the Expression and Activity of NF-κB in Synovium of Collagen-induced Arthritis Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 25; no. 5; pp. 543 - 545
• Main Author: 涂胜豪 胡永红 曾克勤 张明敏 赖先阳 张玮琛
• Format: Journal Article
• Language: English
• Published: Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 01.09.2005
• Subjects: NF-κB; 关节炎; 动物实验; 基因表达; 胶原质; cytokine; collagen-induced arthritis; NF-κB; triptolide; synovium
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/BF02896012

Summary： The expression and activity of NF-kB in the synovium of collagen-induced arthritis （CIA） rats was detected in order to investigate the possible therapeutic effects of triptolide on rheumatoid arthritis （RA）. The experimental Wistar rat model of CIA was set up by intradermal injection of emulsion of bovine collagen 11 and the successful rate of setting-up models was evaluated by arthritis index （AI）. Rats were grouped randomly into three groups： normal, model and treatment group. The expression of TNF-α and IL-6 in synovial fluid was detected by ELISA, and the expression and activity of NF kB in synovium by immunohistochemistry method and by electrophoretic mobility shift assay （EMSA） respectively. As compared with normal group, the expression of TNF a and IL-6 in synovia （P〈0. 05）, and the expression and activity of NF-kB （P〈0.05） in synovium were increased in model group. There was statistical difference in above-mentioned indexes between model group and treatment group. Triptolide may play a protective role in IRA via downregulating the expression and activity of NF-kB in synovium.