Detection and Potential Abundances of Anammox Bacteria in the Paddy Soil

• Published in: Hanguk hwangyeong nonghak hoeji Vol. 39; no. 1; pp. 26 - 35
• Main Authors: Khanal, Anamika, Lee, Seul, Lee, Ji-Hoon
• Format: Journal Article
• Language: English
• Published: 한국환경농학회 31.03.2020
• Subjects: 농학
• ISSN: 1225-3537; 2233-4173
• DOI: 10.5338/KJEA.2020.39.1.4

BACKGROUND: Microbes that govern a unique biochemical process of oxidizing ammonia into dinitrogen gas, such as anaerobic ammonium oxidation (anammox) have been reported to play a pivotal role in agricultural soils and in oceanic environments. However, limited information for anammox bacterial abundance and distribution in the terrestrial habitats has been known. METHODS AND RESULTS: Phylogenetic and next- generation sequencing analyses of bacterial 16S rRNA gene were performed to examine potential anammox bac-teria in paddy soils. Through clone libraries constructed by using the anammox bacteria-specific primers, some clones showed sequence similarities with Planctomycetes (87% to 99%) and anammox bacteria (94% to 95%). Microbial community analysis for the paddy soils by using Illumina Miseq sequencing of 16S rRNA gene at phylum level was dominated by unclassified Bacteria at 33.2 ± 7.6%, followed by Chloroflexi at 20.4 ± 2.0% and Acidobacteria at 17.0 ± 6.5%. Planctomycetes that anammox bacteria are belonged to was 1.5% (± 0.3) on average from the two paddy soils. CONCLUSION: We suggest evidence of anammox bac-teria in the paddy soil. In addition to the relatively well- known microbial processes for nitrogen-cycle, anammox can be a potential contributor on the cycle in terrestrial en-vironments such as paddy soils. KCI Citation Count: 0