The Brassica rapa Rubber Elongation Factor Promoter Regulates Gene Expression During Seedling Growth in Arabidopsis thaliana and Brassica napus

• Published in: Plant breeding and biotechnology pp. 289 - 300
• Main Authors: 홍준기, 이연희, 임호명, 김진아, 김정선, 이성범, 서은정, 이수인
• Format: Journal Article
• Language: English
• Published: 한국육종학회 30.09.2014
• Subjects: 농학
• ISSN: 2287-9358; 2287-9366
• DOI: 10.9787/PBB.2014.2.3.289

A tissue-specific and developmentally expressed gene was isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis), designated BrREF (B. rapa Rubber elongation factor). BrREF transcripts were expressed at high levels in seedlings and atlow levels in flower buds and roots. To study the activity of this promoter, the 2.2 kb upstream sequence of BrREF gene was fused toa β-glucuronidase (GUS) reporter gene and was introduced into Arabidopsis thaliana and B. napus by Agrobacterium-mediatedtransformation. Strong expression of GUS driven by the BrREF promoter was detected in the cotyledons and hypocotyls of transgenicplant seedlings, but GUS expression was weak in roots, excluding the root tips. GUS expression in the cotyledons and hypocotylsdecreased dramatically as the seedlings matured and was not detected in the tissues of mature plants. During floral development, GUSexpression was observed in immature anthers. These findings suggest that the BrREF promoter can modulate the tissue-specific anddevelopmental expression of gene at the early stages of growth and development. KCI Citation Count: 1