Anti-aging Effect of Mixed Extract from Medicinal Herbs

• Published in: Asian Journal of Beauty and Cosmetology Vol. 19; no. 4; pp. 679 - 692
• Main Authors: Lee, Soyoun, An, Hongyan, Kim, Woosoo, Lu, Xinxin, Jeon, Hyanghwa, Park, Han Woong, Ha, Jeongcheol, Cho, Jinhun
• Format: Journal Article
• Language: English
• Published: 한국피부과학연구원 01.12.2021
• Subjects: 미용
• ISSN: 2466-2046; 2466-2054
• DOI: 10.20402/ajbc.2021.0243

Purpose: This study was conducted to confirm the applicability of a mixed herbal extract (MHE) as an anti-aging cosmetic ingredient by investigating its skin anti-aging activities in vitro and in vivo.Methods: In this study, we prepared MHE using an ultrasonic extraction containing Forsythiae fructus, Tribuli fructus, Solomon’s seal, Siberian ginseng, Ponciri fructus and Ginseng. We investigated the anti-aging effect of the MHE for skin in dermal fibroblasts. The anti-aging activity was determined by the type I collagen synthesis levels. Matrix metalloproteinase-1 (MMP1) and tissue inhibitor of metalloproteinases 1 (TIMP1) mRNA levels were measured by qRT-PCR. MMP1 protein levels were evaluated by blotting analysis. Clinical tests of skin moisture, elasticity, texture, and wrinkles were performed using cosmetics containing 1% MHE.Results: The MHE induced the upregulation of pro-collagen type I synthesis and TIMP1 mRNA expression. The MHE led to the downregulation of MMP1 mRNA levels and protein levels. Furthermore, after skin application of cosmetics containing 1% MHE, skin hydration, elasticity, texture, and crow’s feet were improved 4 weeks after the treatment.Conclusion: MHE has an anti-aging effect by promoting collagen synthesis and suppressing MMP1 gene expression in vitro, and it has a skin improvement effect in vivo. Therefore, the MHE was shown to have value as a functional cosmetic ingredient.