Comparative study of conventional Papanicolaou and ultrafast Papanicolaou stains in fine needle aspiration samples and body fluids

• Published in: Journal of Pathology of Nepal Vol. 14; no. 2; pp. 2209 - 2212
• Main Authors: Choudhary, Prabesh, Nepal, Niraj, Bhandari, Rashmita, Baral, Ritu, Shrestha, Oshan, Choudhary, Sarita
• Format: Journal Article
• Language: English
• Published: Association of Clinical Pathologists of Nepal 31.12.2024
• Subjects: body fluid; fine needle aspiration cytology; giemsa; hematoxylin and eosin; modified ultrafast papanicolaou stain
• ISSN: 2091-0797; 2091-0908
• DOI: 10.3126/jpn.v14i2.71320

Background: Ultra Papanicolaou stain was introduced as a hybrid of Romanowsky and Papanicolaou stains. It enhances the quality and reduces the time. Materials and Methods: Five hundred and ninety-five fine needle aspiration and body fluid samples were collected. The fine needle aspiration procedure was performed by standard method; smears were fixed in 95% propanol and stained with conventional and ultra Papanicolaou stains. Four parameters were considered and scored (background, cell morphology, nuclear staining, and overall staining pattern). Results: The quality of ultra Papanicolaou stain smears was better than conventional pap stain, and this difference was statistically significant. Conclusions: UltraPap stain, compared to conventional Pap stain, provides an excellent alternative in cytological staining for the evaluation of various organs and fluids for benign and malignant pathology.