Dexamethasone-Loaded PLGA Microspheres Incorporated PLLA/PLGA/PCL Composite Scaffold for Bone Tissue Engineering
The combination of micro-carriers and polymer scaffolds as promising bone grafts have attracted considerable interest in recent decades. The poly （ L-lactic acid ）/poly （ lactic-co-glycoHc acid ）/polycaprolactone （ PLLA/PLGA/PCL ） composite scaffold with porous structure was fabricated by thermally...
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| Published in | 东华大学学报（英文版） Vol. 34; no. 1; pp. 159 - 163 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China
28.02.2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-5220 |
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| Summary: | The combination of micro-carriers and polymer scaffolds as promising bone grafts have attracted considerable interest in recent decades. The poly （ L-lactic acid ）/poly （ lactic-co-glycoHc acid ）/polycaprolactone （ PLLA/PLGA/PCL ） composite scaffold with porous structure was fabricated by thermally induced phase separation （TIPS）. Dexamethasone （DEX） was incorporated into scaffold to prepare the desired composite scaffold. The physicochemiral properties of the prepared composite scaffold were characterized. The morphology of rat bone marrow mesenchymal stem cells （BMSCs） grown on scaffolds was observed using scanning, electron microscope （SEM） and fluorescence microscope. The results showed that the PLLA/PLGA/PCL scaffold had interconnected macropores and biomimetie nanofibrous structure. In addition, DEX can be released from scaffold in a sustained manner. More importantly, DEX loaded composite scaffold can effectively support the proliferation of BMSCs as indicated by fluorescence observation and cell proliferation assay. The results suggested that the prepared PLLA/PLGA/PCL composite scaffold incorporating drug-loaded PLGA microspheres could hold great potential for bone tissue engineering applications. |
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| Bibliography: | The combination of micro-carriers and polymer scaffolds as promising bone grafts have attracted considerable interest in recent decades. The poly （ L-lactic acid ）/poly （ lactic-co-glycoHc acid ）/polycaprolactone （ PLLA/PLGA/PCL ） composite scaffold with porous structure was fabricated by thermally induced phase separation （TIPS）. Dexamethasone （DEX） was incorporated into scaffold to prepare the desired composite scaffold. The physicochemiral properties of the prepared composite scaffold were characterized. The morphology of rat bone marrow mesenchymal stem cells （BMSCs） grown on scaffolds was observed using scanning, electron microscope （SEM） and fluorescence microscope. The results showed that the PLLA/PLGA/PCL scaffold had interconnected macropores and biomimetie nanofibrous structure. In addition, DEX can be released from scaffold in a sustained manner. More importantly, DEX loaded composite scaffold can effectively support the proliferation of BMSCs as indicated by fluorescence observation and cell proliferation assay. The results suggested that the prepared PLLA/PLGA/PCL composite scaffold incorporating drug-loaded PLGA microspheres could hold great potential for bone tissue engineering applications. 31-1920/N composite scaffold; poly （ lactic-co-glycolic acid） （PLGA） microsphere; dexamethasone; bone tissue engineering MIAO Yingke, NIE Wei, WANG Weizhong, ZHOU Xiaojun, HE Chuanglong （ College of Chemistry, Chemical Engineering and Biotechnology , Donghua University, Shanghai 201620, China） |
| ISSN: | 1672-5220 |