Synthesis and pH Sensitive Fluorescent Performance of 2＇-Chloroseminaphthofluorescein with Dual Bands

• Published in: 东华大学学报（英文版） Vol. 34; no. 4; pp. 507 - 511
• Main Author: 葛凤燕 陈立功 杨雪 袁帅
• Format: Journal Article
• Language: English
• Published: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China 31.08.2017; Key Laboratory of Clean Dyeing and Finishing Technology of Zhejiang Province, Shaoxing 312000, China%School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China%College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
• Subjects: fluorescent properties; Stokes shift; unsymmetrical fluorescein derivative; dual bands
• ISSN: 1672-5220
• DOI: 10.3969/j.issn.1672-5220.2017.04.005

A novel unsymmetrical fluorescein derivative substituted by chlorine and naphthol, 2 ＇-chloroseminaphthofluorescein, was prepared by the two-step synthetic method. This method can effectively control the reactive process,so the pure unsymmetrical fluorescein derivative can be obtained in high yield. The new fluorophore exhibited dual absorption bands,dual excitation bands and dual emission bands with long-wavelength,selectively exciting the acidic and basic forms of the fluorophore. Compared with the similar symmetrical fluorescein derivatives such as 2 ＇, 7 ＇-dichlorofluorescein and dinaphtofluorescein, this fluorophore was found to have larger Stokes shift,especially in the basic form. Based on the properties above,this unsymmetrical fluorescein derivative is expected as a good fluorescent probe to be used where the interference exists due to the presence of endogenous fluorescers.