Fluorescence Tracking of Exogenous DNA in Genetic Transformation of the Chinese Oak Silkmoth Antheraea Pernyi via Sperm-Mediated Gene Transfer
Exogenous DNA expressing green fluorescent protein（ GFP） and labeled with fluorescein isothiocyanate（ FITC） was used to transform the Chinese oak silkmoth Antheraea pernyi（ A. pernyi）via sperm-mediated gene transfer（ SMGT）. Sperms entry into the female reproductive system and eggs were observed usin...
Saved in:
| Published in | 东华大学学报（英文版） Vol. 31; no. 4; pp. 391 - 395 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
School of Agriculture, Eastern Liaoning University, Dandong 118000, China%School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China
31.08.2014
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-5220 |
Cover
| Summary: | Exogenous DNA expressing green fluorescent protein（ GFP） and labeled with fluorescein isothiocyanate（ FITC） was used to transform the Chinese oak silkmoth Antheraea pernyi（ A. pernyi）via sperm-mediated gene transfer（ SMGT）. Sperms entry into the female reproductive system and eggs were observed using fluorescence microscopy. The ability of A. pernyi sperms to uptake exogenous DNA was confirmed,and transfer of the exogenous DNA was shown by GFP expression in the transgenic eggs. Our result suggested that SMGT could also be used to directly generate transgenic A. pernyi expressing functional genes of interest. |
|---|---|
| Bibliography: | 31-1920/N LIU Dan-mei, LI Wen-li （1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China 2 School of Agriculture, Eastern Liaoning University, Dandong 118000, China） Antheraea pernyi（A.pernyi） exogenous DNA fluorescein isothiocyanate（FITC） label green fluorescent protein（GFP） sperm-mediated gene transfer（SMGT） Exogenous DNA expressing green fluorescent protein（ GFP） and labeled with fluorescein isothiocyanate（ FITC） was used to transform the Chinese oak silkmoth Antheraea pernyi（ A. pernyi）via sperm-mediated gene transfer（ SMGT）. Sperms entry into the female reproductive system and eggs were observed using fluorescence microscopy. The ability of A. pernyi sperms to uptake exogenous DNA was confirmed,and transfer of the exogenous DNA was shown by GFP expression in the transgenic eggs. Our result suggested that SMGT could also be used to directly generate transgenic A. pernyi expressing functional genes of interest. |
| ISSN: | 1672-5220 |