Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adso...

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Published in东华大学学报（英文版） Vol. 34; no. 1; pp. 111 - 117
Main Author 杨慧琴 陈天翔 刘琳 陈德强 李树白 权静 朱利民 聂华丽
Format Journal Article
LanguageEnglish
Published College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China%College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China%Changzhou Institute of Engineering Technology,Changzhou 213164,China 28.02.2017
Key Laboratory of Textile Science & Technology,Ministry of Education,Donghua University,Shanghai 201620,China
Subjects
adsorption
response surface methodology
affinity precipitation
papain
heptapeptide
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ISSN1672-5220

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Summary:A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adsorption. Finally, the adsorption conditions were optimized by response surface methodology. The results indicated that the optimum adsorption conditions were determined as initial pH 6.9, temperature 40℃, and the adsorption capacity of CHM for papain was found to be 55.52 mg/g. Moreover, the papain was purified 78- fold in a single step determined by affinity precipitation. More than 87 % of the adsorbed papain was desorbed using the eluent solventtriethylarnine at pH 7.4. The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.
Bibliography:31-1920/N
YANG Huiqin 1,2, CHEN Tianxiang 2, LIU Lin 2, CHEN Deqiang 2, LI Shubai3, QUAN Jing2, ZHU Limin 2, NIE Huali1,2（1 Key Laboratory of Textile Science ＆ Technology, Ministry of Education, Donghua University, Shanghai 201620, China 2 College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China 3 Changzhou Institute of Engineering Technology, Changzhau 213164, China）
heptapeptide; papain; response surface methodology;adsorption; affinity precipitation
A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adsorption. Finally, the adsorption conditions were optimized by response surface methodology. The results indicated that the optimum adsorption conditions were determined as initial pH 6.9, temperature 40℃, and the adsorption capacity of CHM for papain was found to be 55.52 mg/g. Moreover, the papain was purified 78- fold in a single step determined by affinity precipitation. More than 87 % of the adsorbed papain was desorbed using the eluent solventtriethylarnine at pH 7.4. The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.
ISSN:1672-5220