环糊精功能化多壁碳纳米管对芒柄花素的装载及其细胞毒性研究

利用羟丙基-β-环糊精（HP-β-CD）修饰羧基化多壁碳纳米管（CD-MWCNTs）,通过红外光谱证明了CDMWCNTs的成功合成,并经溶液共混法将CD-MWCNTs装载芒柄花素（FMN）。选用激光粒径仪,X射线衍射、扫描电镜对载药CD-MWCNTs（CD-MWCNTs-FMN）进行表征,并考察其释药特性。采用水溶性四唑盐试剂（WST-1）测定CD-MWCNTs-FMN的抗肿瘤活性。研究结果表明CD-MWCNTs对FMN的包封率为（50.60±1.92）%,载药率为（7.20±0.98）%。FMN在CD-MWCNTs的释放行为具有pH值依赖。细胞毒性结果表明载药CD-MWCNTs的抗肿瘤活性强...

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Bibliographic Details
Published in功能材料 Vol. 49; no. 1; pp. 1071 - 1077
Main Author 刘晓红;黄泽贤;许丹翘;廖灿城;许昕浩;郭波红
Format Journal Article
LanguageChinese
Published 广东药科大学 药学院,广州,510006 30.01.2018
Subjects
细胞毒性
羟丙基-β-环糊精
羧基化多壁碳纳米管
芒柄花素
释放
芒柄花素
carboxylic multiwalled carbon nanotubes
释放
formononetin
release
cyto-toxicity
hydroxypropyl-β-cyclodextrin
羟丙基-β-环糊精
细胞毒性
羧基化多壁碳纳米管
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ISSN1001-9731
DOI10.3969/j.issn.1001-9731.2018.01.014

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Summary:利用羟丙基-β-环糊精（HP-β-CD）修饰羧基化多壁碳纳米管（CD-MWCNTs）,通过红外光谱证明了CDMWCNTs的成功合成,并经溶液共混法将CD-MWCNTs装载芒柄花素（FMN）。选用激光粒径仪,X射线衍射、扫描电镜对载药CD-MWCNTs（CD-MWCNTs-FMN）进行表征,并考察其释药特性。采用水溶性四唑盐试剂（WST-1）测定CD-MWCNTs-FMN的抗肿瘤活性。研究结果表明CD-MWCNTs对FMN的包封率为（50.60±1.92）%,载药率为（7.20±0.98）%。FMN在CD-MWCNTs的释放行为具有pH值依赖。细胞毒性结果表明载药CD-MWCNTs的抗肿瘤活性强于游离药物。这说明环糊精修饰的多壁碳纳米管是一种良好的缓释性载药系统,并且能提高芒柄花素的抗肿瘤活性。
Bibliography:The carboxylic multiwalled carbon nanotubes were modified by hydroxypropyl-β-cyclodextrin to prepare CD-MWCNTs.According to the result of Fourier-transform infrared spectrometry（FT-IR）,HP-β-CD has been grafted to MWCNTs-COOH successfully.Then CD-MWCNTs were non-covalently conjugated with FMN,and laser particle size analyzer,X-ray diffractometry（XRD）and scanning electron microscopy（SEM）were used to characterize the compounds.The drug release characteristics were investigated.The anticancer activity of CD-MWCNTs-FMN was determined using a water-soluble tetrazolium salt reagent（WST-1）.The results demonstrated that the encapsulation efficiency of CD-MWCNTs for FMN was（50.60±1.92）% and the drug loading capacity was（7.20±0.98）%.In vitro release studies showed that the drug release rate under an acidic condition（pH 5.5）was lower than that under physiological condition（pH 7.4）,which indicated a pH dependence.The cytotoxicity assay showed that the anti-cancer activity of CD-MWCNTs-FMN was stronger than that of free dr
ISSN:1001-9731
DOI:10.3969/j.issn.1001-9731.2018.01.014