电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究

观察X射线辐射对小鼠骨髓树突状免疫细胞（Dendritic Cells,DCs）的影响。利用X射线对小鼠骨髓树突状免疫细胞进行不同剂量的照射,流式细胞术检测小鼠骨髓DCs的凋亡比例、CCK-8方法检测DCs免疫能力的变化情况,利用蛋白抑制剂对JAK2/STAT3信号转导通路进行抑制,探讨可能的分子机理。与未照射组比较,X射线照射0.5 Gy能够诱导小鼠骨髓DCs抗原递呈和抑制肿瘤细胞活性能力下降,凋亡比例增加,JAK2、STAT和Caspase-3蛋白表达显著增加。X射线能够诱导小鼠骨髓DCs凋亡增加,从而导致细胞免疫能力下降,JAK2/STAT3/Caspase-3通路可能是参与X射线诱导D...

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Bibliographic Details
Published in核技术 Vol. 41; no. 3; pp. 38 - 43
Main Author 张红;罗冰芝;徐凤兰;胡晓娅;张哲;刘小琴
Format Journal Article
LanguageChinese
Published 甘肃省武威肿瘤医院 武威 733000 10.03.2018
中国科学院近代物理研究所 兰州 730000%甘肃省武威肿瘤医院 武威 733000
Subjects
树突状免疫细胞;X射线;凋亡;JAK2/STAT3/Caspase-3信号通路
Dendritic cells
JAK2/STAT3/Caspase-3信号通路
树突状免疫细胞
JAK2/STAT3/Caspase-3 signal pathway
X-ray
凋亡
X射线
Apoptosis
Online AccessGet full text
ISSN0253-3219
DOI10.11889/j.0253-3219.2018.hjs.41.030301

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Summary:观察X射线辐射对小鼠骨髓树突状免疫细胞（Dendritic Cells,DCs）的影响。利用X射线对小鼠骨髓树突状免疫细胞进行不同剂量的照射,流式细胞术检测小鼠骨髓DCs的凋亡比例、CCK-8方法检测DCs免疫能力的变化情况,利用蛋白抑制剂对JAK2/STAT3信号转导通路进行抑制,探讨可能的分子机理。与未照射组比较,X射线照射0.5 Gy能够诱导小鼠骨髓DCs抗原递呈和抑制肿瘤细胞活性能力下降,凋亡比例增加,JAK2、STAT和Caspase-3蛋白表达显著增加。X射线能够诱导小鼠骨髓DCs凋亡增加,从而导致细胞免疫能力下降,JAK2/STAT3/Caspase-3通路可能是参与X射线诱导DCs凋亡的机制之一。
Bibliography:ZHANG Hong1,2, LUO Bingzhi1, XU Fenglan1, HU Xiaoya1, ZHANG Zhe1, LIU Xiaoqin1 （1.Gansu Wuwei Tumor Hostipal, Wuwei 733000, China;2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China）
31-1342/TL
Dendritic cells;X-ray;Apoptosis;JAK2/STAT3/Caspase-3 signal pathway
[Background] Radiation would induce apoptosis of cells. [Purpose] This study aims to observe the effect of irradiation on bone marrow dendritic cells（BMDCs） in mice. [Methods] The BMDCs were irradiated by X-ray to absorbed doses of 0.5 Gy or 1.0 Gy. The apoptosis and immunity ability of DCs were measured by flow cytometry and CCK-8. Protein expressions were determined by Western blot. The JAK2/STAT3 signal transduction pathway was inhibited by protein inhibitors. [Results] The dysfunction of BMDCs induced by X-ray irradiation was observed. The BMDCs apoptosis percentage increased, and JAK-2, STAT-3, and cleaved Caspase-3 protein expressions were promoted. [Conclusion] The up-regulation of JAK2, STAT3 and cleaved Caspase-3 exp
ISSN:0253-3219
DOI:10.11889/j.0253-3219.2018.hjs.41.030301