The Role of Dopamine Receptors in the Modulation of Mononuclear Phagocytes in Multiple Sclerosis

• Published in: Neuroscience and behavioral physiology Vol. 55; no. 1; pp. 133 - 138
• Main Authors: Lopatina, A. V., Sviridova, A. A., Belousova, O. O., Kuzmina, U. Sh, Melnikov, M. V.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.01.2025; Springer Nature B.V
• Subjects: Behavioral Sciences; Biomedical and Life Sciences; Biomedicine; CD14 antigen; Cell culture; Cytokines; Dopamine; Dopamine D1 receptors; Dopamine D2 receptors; Dopamine receptors; Enzyme-linked immunosorbent assay; IL-1β; Immune response; Immunoregulation; Innate immunity; Interleukin 6; Leukocytes (mononuclear); Lipopolysaccharides; Macrophages; Monocytes; Multiple sclerosis; Neurobiology; Neurosciences; Original Research; Phagocytes; γ-Interferon; monocytes; macrophages; dopamine receptors; neuroimmune interaction; multiple sclerosis
• ISSN: 0097-0549; 1573-899X
• DOI: 10.1007/s11055-025-01763-6

Objective . To study the role of D 1 DR and D 2 DR dopamine receptors in the production of interleukins (IL) IL-6 and IL-1β by monocytes and macrophages in patients with relapsing-remitting multiple sclerosis (MS). Materials and methods . Ten patients with relapsing-remitting MS and 10 healthy subjects were investigated. Levels of IL-6 and IL-1β production were assessed in culture supernatants obtained from CD14 + monocytes or macrophages stimulated with interferon-γ (IFN-γ) and lipopolysaccharide (LPS). The role of dopamine receptors in the regulation of CD14 + monocytes and macrophages was studied by incubating cell samples in the presence of specific D 1 DR or D 2 DR antagonists, after which they were stimulated with IFN-γ/LPS. Cytokine levels in cell culture supernatants were measured by enzyme-linked immunosorbent assay. Results . IL-6 and IL-1β production levels by CD14 + monocytes and macrophages were similar in the two groups. Blockade of D 1 DR suppressed cytokine production by both CD14 + monocytes and macrophages in both groups. In contrast, blockade of D 2 DR enhanced cytokine production by CD14 + monocytes and had no effect on macrophages in both groups. Conclusions . Targeting of dopamine receptors may provide an additional mechanism of immunoregulation in MS able to exert both pro- and anti-inflammatory effects on innate immune response cells.