Effect of Granulocyte Colony-Stimulating Factor Liposomal Form on the Expression of Genes Associated with Chronic Endometritis

• Published in: Journal of evolutionary biochemistry and physiology Vol. 61; no. 4; pp. 1217 - 1226
• Main Authors: Ryzhov, J. R., Obedkova, K. V., Khalenko, V. V., Gzgzyan, A. M., Tapilskaya, N. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2025; Springer Nature B.V
• Subjects: Animal Physiology; Bioavailability; Biochemistry; Biomedical and Life Sciences; Biopsy; Colony-stimulating factor; Embryogenesis; Endometritis; Endometrium; Evolutionary Biology; Experimental Papers; Granulocyte colony-stimulating factor; Granulocytes; Implantation; Interleukin 10; Leukocytes (granulocytic); Leukocytes (neutrophilic); Life Sciences; Reproductive technologies; Transforming growth factor-b1; in vitro fertilization; granulocyte colony-stimulating factor; infertility; chronic endometritis; assisted reproductive technologies
• ISSN: 0022-0930; 1608-3202
• DOI: 10.1134/S0022093025040222

While the classical function of granulocyte colony-stimulating factor (G-CSF) is the regulation of neutrophil growth and differentiation, this cytokine also plays an important role in folliculo- and embryogenesis, embryo implantation and trophoblast invasion, thus arousing significant interest in its application in assisted reproductive technology (ART) programs. It has been established that intrauterine G-CSF administration in ART protocols for patients with chronic endometritis and recurrent implantation failure increases pregnancy rates; however, the pathogenetic mechanisms of its action require further investigation. We have developed a liposomal form of G-CSF, characterized by a higher bioavailability upon intrauterine administration compared to an aqueous G-CSF solution. The study involved 22 infertile patients with a thin endometrium and repeated ART failures, who received therapy for chronic endometritis via intrauterine administration of the liposomal G-CSF form twice a week for 6 months. Using real-time PCR, the expression of TVP23A , IL10 , and TGF-β1 genes in endometrial biopsies was assessed before and after 6 months of treatment with the liposomal G-CSF form. This therapy significantly reduced the expression of the TVP23A gene ( p < 0.001) and increased the expression of IL10 ( p < 0.001) and TGF-β1 ( p < 0.001) genes in the endometrium. A significant ( p < 0.001) increase in endometrial thickness (M-echo) from 5.6 ± 0.6 to 6.8 ± 0.8 mm was observed. The obtained data demonstrate that therapy for chronic endometritis via intrauterine administration of the liposomal G-CSF form for 6 months normalizes the expression of some genes associated with chronic endometrial inflammation.