Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

• Published in: Journal of extracellular vesicles Vol. 7; no. 1; pp. 1535750 - n/a
• Main Authors: Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Andriantsitohaina, Ramaroson, Baharvand, Hossein, Bauer, Natalie N, Baxter, Amy A, Beckham, Carla, Bielska, Ewa, Boireau, Wilfrid, Bongiovanni, Antonella, Brisson, Alain, Broekman, Marike LD, Bryl-Górecka, Paulina, Buch, Shilpa, Bussolati, Benedetta, Caruso, Sarah, Clayton, Aled, Cocucci, Emanuele, de Candia, Paola, Demaret, Tanguy, Dourado, Mauricio R, Falcón-Pérez, Juan M, Försönits, András, Frelet-Barrand, Annie, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gaudin, Raphael, Handberg, Aase, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F, Inal, Jameel M, Ivanova, Alena, Jackson, Hannah K, Jayachandran, Muthuvel, Jiang, Lanzhou, Kalluri, Raghu, Kaur, Sukhbir, Kierulf, Peter, Kim, Kwang Pyo, Krasemann, Susanne, Kurochkin, Igor V, Langevin, Scott M, Lázaro-Ibáñez, Elisa, Lee, Yi Xin Fiona, Libregts, Sten F, Linē, Aija, Linnemannstöns, Karen, Lombard, Catherine A, Lukomska, Barbara, Marcilla, Antonio, Mathivanan, Suresh, McVey, Mark J, Mertens, Inge, Morhayim, Jess, Mullier, François, Nawaz, Muhammad, Nimrichter, Leonardo, Noren Hooten, Nicole, Olivier, Martin, Ortiz, Luis A, Ostrowski, Matias, Pegtel, D. Michiel, Perut, Francesca, Polakovicova, Iva, Pulliam, Lynn, Regev-Rudzki, Neta, Rouschop, Kasper MA, Rughetti, Aurelia, Saá, Paula, Saul, Meike J, Schøyen, Tine Hiorth, Shahaj, Eriomina, Sharma, Shivani, Shekari, Faezeh, Siljander, Pia R-M, Skowronek, Agata, Soares, Rodrigo Pedro, Stoorvogel, Willem, Stott, Shannon L, Tixeira, Rochelle, Toh, Wei Seong, Tomasini, Richard, van Herwijnen, Martijn JC, van Niel, Guillaume, van Wijnen, Andre J, Vasconcelos, M Helena, Veit, Tiago D, Viñas, Jose L, Visnovitz, Tamás, Wehman, Ann M, Weiss, Daniel J, Wheelock, Asa M, Xagorari, Angeliki, Xander, Patricia, Zhang, Jian-ye, Zheutlin, Alexander R, Zickler, Antje M
• Format: Journal Article Web Resource
• Language: English
• Published: United States Taylor & Francis 2018; John Wiley & Sons, Inc; Co-Action Publishing; Informa UK Limited; Taylor and Francis Ltd; Wiley
• Subjects: Apoptosis; Basic Medicine; Biosynthesis; Cancer; Cell and Molecular Biology; Cell Biology; Cell interaction; Cell membranes; Cell- och molekylärbiologi; Cellbiologi; Cellular Biology; Chromatography; ectosomes; Exosomes; Extracellular vesicles; Gram-positive bacteria; guidelines; Histology; Interacció cel·lular; Life Sciences; Lipids; Lipoproteins; Medical and Health Sciences; Medical research; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Membranes cel·lulars; Metastasis; Methods; Microparticles; microvesicles; minimal information requirements; Molecular weight; Physiology; Polls & surveys; Position Paper; Proteins; Reproducibility; rigor; Sciences du vivant; Scientists; standardization; extracellular vesicles; rigor; exosomes; reproducibility; guidelines; microparticles; standardization; microvesicles; minimal information requirements; ectosomes
• ISSN: 2001-3078; 2001-3078
• DOI: 10.1080/20013078.2018.1535750

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.