The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
ObjectivesThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets...
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| Published in | Journal for immunotherapy of cancer Vol. 8; no. 1; p. e000155 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
BMJ Publishing Group Ltd
01.05.2020
BMJ Publishing Group LTD BMJ Publishing Group |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2051-1426 2051-1426 |
| DOI | 10.1136/jitc-2019-000155 |
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| Abstract | ObjectivesThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.MethodsThe Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.ResultsRepresentative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.ConclusionsmIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. |
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| AbstractList | The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.
The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.
Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.
mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.OBJECTIVESThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.METHODSThe Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.RESULTSRepresentative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.CONCLUSIONSmIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. ObjectivesThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.MethodsThe Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.ResultsRepresentative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.ConclusionsmIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. |
| Author | Schenck, Emanuel Rebelatto, Marlon C Hedvat, Cyrus V Tetzlaff, Michael T Greenwald, Noah F Stack, Edward C Engle, Elizabeth L von Loga, Katharina Schalper, Kurt A Hollmann, Travis J Greenbaum, Shirley Parra, Edwin R Ferreira, Cláudia S Lako, Ana Korski, Konstanty Steele, Keith E Juco, Jonathan Taube, Janis M Gnjatic, Sacha Rimm, David L Wistuba, Ignacio I Rodriguez-Canales, Jaime Angelo, Michael Surace, Michael J Rodig, Scott J Bifulco, Carlo B Akturk, Guray |
| AuthorAffiliation | 6 Dermatopathology , Memorial Sloan-Kettering Cancer Center , New York , New York , USA 10 Department of Pathology , Yale University School of Medicine , New Haven , Connecticut , USA 3 Department of Pathology , Stanford University School of Medicine , Palo Alto , California , USA 9 AstraZeneca , Gaithersburg , Maryland , USA 8 Department of Translational Molecular Pathology , University of Texas MD Anderson Cancer Center , Houston , Texas , USA 13 Dana-Farber/Brigham and Women's Cancer Center , Boston , Massachusetts , USA 4 Cancer Biology Program , Stanford University School of Medicine , Palo Alto , California , USA 11 Jounce Therapeutics Inc , Cambridge , Massachusetts , USA 15 Department of Pathology , University of Texas MD Anderson Cancer Center , Houston , Texas , USA 5 Bristol-Myers Squibb , New York , New York City , USA 7 Merck & Co Inc , Kenilworth , New Jersey , USA 14 Harvard Medical School , Boston , Massachusetts , USA 16 Biomedical Research Centre , Royal Marsden NHS Foundation Tr |
| AuthorAffiliation_xml | – name: 1 Department of Dermatology , Johns Hopkins School of Medicine, Bloomberg~Kimmel Institute for Cancer Immunotherapy , Baltimore , Maryland , USA – name: 8 Department of Translational Molecular Pathology , University of Texas MD Anderson Cancer Center , Houston , Texas , USA – name: 17 Providence Portland Medical Center , Portland , Oregon , USA – name: 12 Pharma Research and Early Development (pRED) , Roche Innovation Center Munich , Penzberg , Germany – name: 13 Dana-Farber/Brigham and Women's Cancer Center , Boston , Massachusetts , USA – name: 14 Harvard Medical School , Boston , Massachusetts , USA – name: 9 AstraZeneca , Gaithersburg , Maryland , USA – name: 11 Jounce Therapeutics Inc , Cambridge , Massachusetts , USA – name: 16 Biomedical Research Centre , Royal Marsden NHS Foundation Trust , London , UK – name: 15 Department of Pathology , University of Texas MD Anderson Cancer Center , Houston , Texas , USA – name: 10 Department of Pathology , Yale University School of Medicine , New Haven , Connecticut , USA – name: 3 Department of Pathology , Stanford University School of Medicine , Palo Alto , California , USA – name: 6 Dermatopathology , Memorial Sloan-Kettering Cancer Center , New York , New York , USA – name: 2 Tisch Cancer Institute , Icahn School of Medicine at Mount Sinai , New York , New York City , USA – name: 4 Cancer Biology Program , Stanford University School of Medicine , Palo Alto , California , USA – name: 7 Merck & Co Inc , Kenilworth , New Jersey , USA – name: 5 Bristol-Myers Squibb , New York , New York City , USA |
| Author_xml | – sequence: 1 givenname: Janis M surname: Taube fullname: Taube, Janis M email: jtaube1@jhmi.edu organization: Department of Dermatology, Johns Hopkins School of Medicine, Bloomberg~Kimmel Institute for Cancer Immunotherapy, Baltimore, Maryland, USA – sequence: 2 givenname: Guray orcidid: 0000-0001-7032-8085 surname: Akturk fullname: Akturk, Guray organization: Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York City, USA – sequence: 3 givenname: Michael surname: Angelo fullname: Angelo, Michael organization: Department of Pathology, Stanford University School of Medicine, Palo Alto, California, USA – sequence: 4 givenname: Elizabeth L surname: Engle fullname: Engle, Elizabeth L organization: Department of Dermatology, Johns Hopkins School of Medicine, Bloomberg~Kimmel Institute for Cancer Immunotherapy, Baltimore, Maryland, USA – sequence: 5 givenname: Sacha surname: Gnjatic fullname: Gnjatic, Sacha organization: Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York City, USA – sequence: 6 givenname: Shirley surname: Greenbaum fullname: Greenbaum, Shirley organization: Department of Pathology, Stanford University School of Medicine, Palo Alto, California, USA – sequence: 7 givenname: Noah F surname: Greenwald fullname: Greenwald, Noah F organization: Cancer Biology Program, Stanford University School of Medicine, Palo Alto, California, USA – sequence: 8 givenname: Cyrus V surname: Hedvat fullname: Hedvat, Cyrus V organization: Bristol-Myers Squibb, New York, New York City, USA – sequence: 9 givenname: Travis J surname: Hollmann fullname: Hollmann, Travis J organization: Dermatopathology, Memorial Sloan-Kettering Cancer Center, New York, New York, USA – sequence: 10 givenname: Jonathan surname: Juco fullname: Juco, Jonathan organization: Merck & Co Inc, Kenilworth, New Jersey, USA – sequence: 11 givenname: Edwin R surname: Parra fullname: Parra, Edwin R organization: Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA – sequence: 12 givenname: Marlon C surname: Rebelatto fullname: Rebelatto, Marlon C organization: AstraZeneca, Gaithersburg, Maryland, USA – sequence: 13 givenname: David L orcidid: 0000-0001-5820-4397 surname: Rimm fullname: Rimm, David L organization: Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA – sequence: 14 givenname: Jaime surname: Rodriguez-Canales fullname: Rodriguez-Canales, Jaime organization: AstraZeneca, Gaithersburg, Maryland, USA – sequence: 15 givenname: Kurt A surname: Schalper fullname: Schalper, Kurt A organization: Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA – sequence: 16 givenname: Edward C surname: Stack fullname: Stack, Edward C organization: Jounce Therapeutics Inc, Cambridge, Massachusetts, USA – sequence: 17 givenname: Cláudia S surname: Ferreira fullname: Ferreira, Cláudia S organization: Pharma Research and Early Development (pRED), Roche Innovation Center Munich, Penzberg, Germany – sequence: 18 givenname: Konstanty surname: Korski fullname: Korski, Konstanty organization: Pharma Research and Early Development (pRED), Roche Innovation Center Munich, Penzberg, Germany – sequence: 19 givenname: Ana surname: Lako fullname: Lako, Ana organization: Harvard Medical School, Boston, Massachusetts, USA – sequence: 20 givenname: Scott J surname: Rodig fullname: Rodig, Scott J organization: Harvard Medical School, Boston, Massachusetts, USA – sequence: 21 givenname: Emanuel surname: Schenck fullname: Schenck, Emanuel organization: AstraZeneca, Gaithersburg, Maryland, USA – sequence: 22 givenname: Keith E surname: Steele fullname: Steele, Keith E organization: AstraZeneca, Gaithersburg, Maryland, USA – sequence: 23 givenname: Michael J surname: Surace fullname: Surace, Michael J organization: AstraZeneca, Gaithersburg, Maryland, USA – sequence: 24 givenname: Michael T surname: Tetzlaff fullname: Tetzlaff, Michael T organization: Department of Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA – sequence: 25 givenname: Katharina surname: von Loga fullname: von Loga, Katharina organization: Biomedical Research Centre, Royal Marsden NHS Foundation Trust, London, UK – sequence: 26 givenname: Ignacio I surname: Wistuba fullname: Wistuba, Ignacio I organization: Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA – sequence: 27 givenname: Carlo B surname: Bifulco fullname: Bifulco, Carlo B organization: Providence Portland Medical Center, Portland, Oregon, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32414858$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/j.ymeth.2014.08.016 10.1177/42.3.7508473 10.1371/journal.pone.0038011 10.1038/nature14011 10.1043/1543-2165-133.5.743 10.1002/cyto.a.20311 10.1097/PAI.0b013e31804c7283 10.1146/annurev-med-092012-112807 10.7554/eLife.31657 10.1001/jamaoncol.2019.1549 10.1158/1055-9965.EPI-10-0097 10.1200/JCO.2003.11.069 10.1038/s41598-017-13942-8 10.1038/nm.3488 10.1073/pnas.1607836113 10.1002/cne.20839 10.1038/s41591-018-0339-5 10.1016/j.proghi.2006.09.001 10.1038/modpathol.2017.156 10.1038/nmeth.2019 10.1038/nmeth.3995 10.1186/s40425-017-0243-4 10.1016/j.cell.2016.04.019 10.3791/58390 10.1016/j.cell.2018.07.010 10.1016/j.cell.2018.08.039 10.1093/annonc/mdz019 10.1038/s41467-018-06089-1 10.1172/JCI87324 10.1136/jcp.2007.047720 10.1093/annonc/mdy218 10.1109/TBME.2015.2503122 10.5858/arpa.2013-0610-CP 10.1158/1078-0432.CCR-19-0104 10.1369/jhc.2009.953240 10.1038/nature13954 10.1369/jhc.2010.957191 10.1080/14737159.2017.1268533 10.1186/s40425-015-0091-z 10.1016/j.celrep.2017.03.037 10.1002/cne.10858 10.1186/s40425-016-0178-1 10.1093/jnci/dji427 10.1038/nmeth.3337 10.1002/cyto.a.23053 10.5555/850924.851523 10.1177/002215540305100907 10.1158/2159-8290.CD-15-1545 10.1073/pnas.1300136110 10.1369/jhc.6P6945.2006 10.1126/sciadv.aax5851 10.3109/10520290903039078 10.1016/j.immuni.2018.03.023 10.1038/labinvest.2016.148 10.1016/j.cmet.2018.11.014 10.1016/j.cell.2017.08.027 10.1126/sciimmunol.aaf6925 10.1038/nmeth.2869 10.2217/pme.13.42 10.1186/s12885-016-2948-z 10.2144/000113382 10.1109/ICCV.1999.790410 10.1158/1538-7445.SABCS18-LB-318 10.1137/1.9780898718843 10.3791/58390-v |
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| Keywords | tumors image analysis immunology oncology |
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| References | Bordeaux, Welsh, Agarwal (R24) 2010; 48 Toki, Cecchi, Hembrough (R34) 2017; 97 Daud, Loo, Pauli (R12) 2016; 126 (R26) 2003 Remark, Merghoub, Grabe (R41) 2016; 1 Feng, Puri, Moudgil (R18) 2015; 3 Taube, Galon, Sholl (R4) 2018; 31 Chang, Ornatsky, Siddiqui (R47) 2017; 91 Lowe (R48) 1999; 2 Shi, Shi, Taylor (R27) 2011; 59 Ribas, Dummer, Puzanov (R10) 2017; 170 Negoescu, Labat-Moleur, Lorimier (R44) 1994; 42 Parra, Uraoka, Jiang (R5) 2017; 7 Akturk, Sweeney, Remark (R42) 2055; 2020 Bussolati, Leonardo (R31) 2008; 61 Hirsch, Varella-Garcia, Bunn (R40) 2003; 21 Page, Postow, Callahan (R1) 2014; 65 McCabe, Dolled-Filhart, Camp (R35) 2005; 97 Schalper, Rodriguez-Ruiz, Diez-Valle (R6) 2019; 25 Tumeh, Harview, Yearley (R3) 2014; 515 Chifman, Pullikuth, Chou (R16) 2016; 16 Zhao, Uduman, Siu (R63) 2018; 9 Schindelin, Arganda-Carreras, Frise (R50) 2012; 9 Fitzgibbons, Bradley, Fatheree (R38) 2014; 138 Keren, Bosse, Thompson (R64) 2019; 5 Cardona, Saalfeld, Schindelin (R49) 2012; 7 Masucci, Cesano, Hawtin (R21) 2016; 4 Bogen, Vani, Sompuram (R30) 2009; 84 Toki, Merritt, Wong (R68) 2019; 25 Anagnostou, Welsh, Giltnane (R32) 2010; 19 Garini, Young, McNamara (R46) 2006; 69A Thorsson, Gibbs, Brown (R13) 2018; 48 Pirici, Mogoanta, Kumar-Singh (R43) 2009; 57 Hoyt, Roman, Engle (R58) 2019; 79 Gnjatic, Bronte, Brunet (R11) 2017; 5 Goldstein, Hewitt, Taylor (R36) 2007; 15 Surace, DaCosta, Huntley (R57) 2019 Keren, Bosse, Marquez (R61) 2018; 174 Spitzer, Nolan (R20) 2016; 165 Cottrell, Thompson, Forde (R8) 2018; 29 Saper (R23) 2005; 493 Jennings, Van Deerlin, Gulley (R37) 2009; 133 Lotz, Olesch, Müller (R51) 2016; 63 Lin, Izar, Wang (R55) 2018; 7 Saper, Sawchenko, peptides (R22) 2003; 465 Newman, Liu, Green (R15) 2015; 12 Danilova, Wang, Sunshine (R14) 2016; 113 Simultaneous (R67) 1783; 2018 Damond, Engler, Zanotelli (R62) 2019; 29 Chen, Roh, Reuben (R7) 2016; 6 Yamashita (R28) 2007; 41 Goltsev, Samusik, Kennedy-Darling (R54) 2018; 174 Gerdes, Sevinsky, Sood (R56) 2013; 110 Angelo, Bendall, Finck (R59) 2014; 20 Lu, Stein, Rimm (R17) 2019 Boenisch (R29) 2006; 54 Tsujikawa, Kumar, Borkar (R45) 2017; 19 Dillon, D"Aquila, Reynolds (R53) 1998; 152 Gustavson, Rimm, Dolled-Filhart (R33) 2013; 10 Giesen, Wang, Schapiro (R60) 2014; 11 Herbst, Soria, Kowanetz (R2) 2014; 515 Stein, Soni, Danilova (R9) 2019; 30 Stack, Wang, Roman (R19) 2014; 70 Hasui, Takatsuka, Sakamoto (R65) 2003; 51 Uhlen, Bandrowski, Carr (R25) 2016; 13 Tsang, Xue, Koh (R66) 2017; 17 Stack, Wang, Roman 2014; 70 Toki, Merritt, Wong 2019; 25 Saper 2005; 493 Hirsch, Varella-Garcia, Bunn 2003; 21 Anagnostou, Welsh, Giltnane 2010; 19 Taube, Galon, Sholl 2018; 31 Pirici, Mogoanta, Kumar-Singh 2009; 57 Remark, Merghoub, Grabe 2016; 1 Page, Postow, Callahan 2014; 65 Cardona, Saalfeld, Schindelin 2012; 7 Hasui, Takatsuka, Sakamoto 2003; 51 Gustavson, Rimm, Dolled-Filhart 2013; 10 Lowe 1999; 2 Chang, Ornatsky, Siddiqui 2017; 91 Tsang, Xue, Koh 2017; 17 Fitzgibbons, Bradley, Fatheree 2014; 138 Schalper, Rodriguez-Ruiz, Diez-Valle 2019; 25 Chen, Roh, Reuben 2016; 6 Parra, Uraoka, Jiang 2017; 7 Spitzer, Nolan 2016; 165 Boenisch 2006; 54 Ribas, Dummer, Puzanov 2017; 170 Keren, Bosse, Thompson 2019; 5 Stein, Soni, Danilova 2019; 30 Jennings, Van Deerlin, Gulley 2009; 133 Danilova, Wang, Sunshine 2016; 113 Gerdes, Sevinsky, Sood 2013; 110 Tumeh, Harview, Yearley 2014; 515 Bussolati, Leonardo 2008; 61 Uhlen, Bandrowski, Carr 2016; 13 Chifman, Pullikuth, Chou 2016; 16 Masucci, Cesano, Hawtin 2016; 4 McCabe, Dolled-Filhart, Camp 2005; 97 Saper, Sawchenko, peptides 2003; 465 Simultaneous 1783; 2018 Garini, Young, McNamara 2006; 69A Lin, Izar, Wang 2018; 7 Herbst, Soria, Kowanetz 2014; 515 Surace, DaCosta, Huntley 2019 Gnjatic, Bronte, Brunet 2017; 5 Keren, Bosse, Marquez 2018; 174 Goltsev, Samusik, Kennedy-Darling 2018; 174 Shi, Shi, Taylor 2011; 59 2003 Lotz, Olesch, Müller 2016; 63 Giesen, Wang, Schapiro 2014; 11 Yamashita 2007; 41 Toki, Cecchi, Hembrough 2017; 97 Dillon, D'Aquila, Reynolds 1998; 152 Lu, Stein, Rimm 2019 Tsujikawa, Kumar, Borkar 2017; 19 Zhao, Uduman, Siu 2018; 9 Schindelin, Arganda-Carreras, Frise 2012; 9 Negoescu, Labat-Moleur, Lorimier 1994; 42 Hoyt, Roman, Engle 2019; 79 Bogen, Vani, Sompuram 2009; 84 Thorsson, Gibbs, Brown 2018; 48 Bordeaux, Welsh, Agarwal 2010; 48 Daud, Loo, Pauli 2016; 126 Newman, Liu, Green 2015; 12 Angelo, Bendall, Finck 2014; 20 Feng, Puri, Moudgil 2015; 3 Goldstein, Hewitt, Taylor 2007; 15 Damond, Engler, Zanotelli 2019; 29 Cottrell, Thompson, Forde 2018; 29 Akturk, Sweeney, Remark 2055; 2020 2024110222451197000_8.1.e000155.10 2024110222451197000_8.1.e000155.54 2024110222451197000_8.1.e000155.11 2024110222451197000_8.1.e000155.55 2024110222451197000_8.1.e000155.52 2024110222451197000_8.1.e000155.14 2024110222451197000_8.1.e000155.58 2024110222451197000_8.1.e000155.15 2024110222451197000_8.1.e000155.59 2024110222451197000_8.1.e000155.12 2024110222451197000_8.1.e000155.56 2024110222451197000_8.1.e000155.13 2024110222451197000_8.1.e000155.57 2024110222451197000_8.1.e000155.50 Lowe (2024110222451197000_8.1.e000155.48) 1999; 2 Remark (2024110222451197000_8.1.e000155.41) 2016; 1 2024110222451197000_8.1.e000155.18 2024110222451197000_8.1.e000155.19 2024110222451197000_8.1.e000155.17 Gustavson (2024110222451197000_8.1.e000155.33) 2013; 10 2024110222451197000_8.1.e000155.43 2024110222451197000_8.1.e000155.44 Chang (2024110222451197000_8.1.e000155.47) 2017; 91 Stein (2024110222451197000_8.1.e000155.9) 2019; 30 2024110222451197000_8.1.e000155.40 2024110222451197000_8.1.e000155.8 2024110222451197000_8.1.e000155.7 2024110222451197000_8.1.e000155.4 2024110222451197000_8.1.e000155.3 2024110222451197000_8.1.e000155.6 Tsujikawa (2024110222451197000_8.1.e000155.45) 2017; 19 2024110222451197000_8.1.e000155.2 Akturk (2024110222451197000_8.1.e000155.42) 2055; 2020 2024110222451197000_8.1.e000155.1 Chifman (2024110222451197000_8.1.e000155.16) 2016; 16 2024110222451197000_8.1.e000155.32 2024110222451197000_8.1.e000155.30 2024110222451197000_8.1.e000155.31 2024110222451197000_8.1.e000155.36 2024110222451197000_8.1.e000155.37 2024110222451197000_8.1.e000155.34 2024110222451197000_8.1.e000155.35 Cardona (2024110222451197000_8.1.e000155.49) 2012; 7 Dillon (2024110222451197000_8.1.e000155.53) 1998; 152 2024110222451197000_8.1.e000155.38 2024110222451197000_8.1.e000155.39 Tsang (2024110222451197000_8.1.e000155.66) 2017; 17 2024110222451197000_8.1.e000155.21 2024110222451197000_8.1.e000155.65 2024110222451197000_8.1.e000155.22 Garini (2024110222451197000_8.1.e000155.46) 2006; 69A 2024110222451197000_8.1.e000155.20 2024110222451197000_8.1.e000155.64 2024110222451197000_8.1.e000155.25 2024110222451197000_8.1.e000155.23 2024110222451197000_8.1.e000155.24 2024110222451197000_8.1.e000155.68 Zhao (2024110222451197000_8.1.e000155.63) 2018; 9 2024110222451197000_8.1.e000155.61 2024110222451197000_8.1.e000155.62 2024110222451197000_8.1.e000155.60 cr-split#-2024110222451197000_8.1.e000155.26.2 cr-split#-2024110222451197000_8.1.e000155.26.1 Simultaneous (2024110222451197000_8.1.e000155.67) 1783; 2018 Parra (2024110222451197000_8.1.e000155.5) 2017; 7 2024110222451197000_8.1.e000155.29 2024110222451197000_8.1.e000155.27 2024110222451197000_8.1.e000155.28 Lotz (2024110222451197000_8.1.e000155.51) 2016; 63 32499296 - J Immunother Cancer. 2020 Jun;8(1):e000155corr1. doi: 10.1136/jitc-2019-000155corr1 |
| References_xml | – volume: 70 start-page: 46 year: 2014 ident: R19 article-title: Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis publication-title: Methods doi: 10.1016/j.ymeth.2014.08.016 – volume: 42 start-page: 433 year: 1994 ident: R44 article-title: F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence publication-title: J Histochem Cytochem. doi: 10.1177/42.3.7508473 – volume: 7 year: 2012 ident: R49 article-title: TrakEM2 software for neural circuit reconstruction publication-title: PLoS One doi: 10.1371/journal.pone.0038011 – volume: 515 start-page: 563 year: 2014 ident: R2 article-title: Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients publication-title: Nature doi: 10.1038/nature14011 – volume: 133 start-page: 743 year: 2009 ident: R37 article-title: Recommended principles and practices for validating clinical molecular pathology tests publication-title: Arch Pathol Lab Med doi: 10.1043/1543-2165-133.5.743 – volume: 69A start-page: 735 year: 2006 ident: R46 article-title: Spectral imaging: principles and applications publication-title: Cytometry Part A doi: 10.1002/cyto.a.20311 – volume: 15 start-page: 124 year: 2007 ident: R36 article-title: Recommendations for improved standardization of immunohistochemistry publication-title: Appl Immunohistochem Mol Morphol doi: 10.1097/PAI.0b013e31804c7283 – volume: 65 start-page: 185 year: 2014 ident: R1 article-title: Immune modulation in cancer with antibodies publication-title: Annu Rev Med doi: 10.1146/annurev-med-092012-112807 – volume: 7 year: 2018 ident: R55 article-title: Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes publication-title: eLife doi: 10.7554/eLife.31657 – year: 2019 ident: R17 article-title: Comparison of Biomarker Modalities for Predicting Response to PD-(L)1 Checkpoint Blockade: A Systematic Review and Meta-Analysisin press publication-title: JAMA Oncol doi: 10.1001/jamaoncol.2019.1549 – volume: 2020 start-page: 497 year: 2055 ident: R42 article-title: Multiplexed immunohistochemical consecutive staining on single slide (MICSSS): multiplexed chromogenic IHC assay for high-dimensional tissue analysis publication-title: Methods Mol Biol – volume: 19 start-page: 982 year: 2010 ident: R32 article-title: Analytic variability in immunohistochemistry biomarker studies. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology publication-title: Cancer Epidemiol Biomarkers Prev doi: 10.1158/1055-9965.EPI-10-0097 – volume: 21 start-page: 3798 year: 2003 ident: R40 article-title: Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis publication-title: J Clin Oncol doi: 10.1200/JCO.2003.11.069 – volume: 7 year: 2017 ident: R5 article-title: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues publication-title: Sci Rep doi: 10.1038/s41598-017-13942-8 – volume: 20 start-page: 436 year: 2014 ident: R59 article-title: Multiplexed ion beam imaging of human breast tumors publication-title: Nat Med doi: 10.1038/nm.3488 – volume: 113 start-page: E7769 year: 2016 ident: R14 article-title: Association of PD-1/PD-L axis expression with cytolytic activity, mutational load, and prognosis in melanoma and other solid tumors publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1607836113 – volume: 493 start-page: 477 year: 2005 ident: R23 article-title: An open letter to our readers on the use of antibodies publication-title: J Comp Neurol doi: 10.1002/cne.20839 – volume: 25 start-page: 470 year: 2019 ident: R6 article-title: Neoadjuvant nivolumab modifies the tumor immune microenvironment in resectable glioblastoma publication-title: Nat Med doi: 10.1038/s41591-018-0339-5 – volume: 41 start-page: 141 year: 2007 ident: R28 article-title: Heat-Induced antigen retrieval: mechanisms and application to histochemistry publication-title: Prog Histochem Cytochem doi: 10.1016/j.proghi.2006.09.001 – volume: 31 start-page: 214 year: 2018 ident: R4 article-title: Implications of the tumor immune microenvironment for staging and therapeutics publication-title: Mod Pathol doi: 10.1038/modpathol.2017.156 – volume: 9 start-page: 676 year: 2012 ident: R50 article-title: Fiji: an open-source platform for biological-image analysis publication-title: Nat Methods doi: 10.1038/nmeth.2019 – volume: 13 start-page: 823 year: 2016 ident: R25 article-title: A proposal for validation of antibodies publication-title: Nat Methods doi: 10.1038/nmeth.3995 – volume: 5 start-page: 44 year: 2017 ident: R11 article-title: Identifying baseline immune-related biomarkers to predict clinical outcome of immunotherapy publication-title: J Immunother Cancer doi: 10.1186/s40425-017-0243-4 – volume: 165 start-page: 780 year: 2016 ident: R20 article-title: Mass cytometry: single cells, many features publication-title: Cell doi: 10.1016/j.cell.2016.04.019 – year: 2019 ident: R57 article-title: Automated multiplex immunofluorescence panel for Immuno-oncology studies on formalin-fixed carcinoma tissue specimens publication-title: J Vis Exp doi: 10.3791/58390 – volume: 174 start-page: 968 year: 2018 ident: R54 article-title: Deep profiling of mouse splenic architecture with Codex multiplexed imaging publication-title: Cell doi: 10.1016/j.cell.2018.07.010 – volume: 174 start-page: 1373 year: 2018 ident: R61 article-title: A structured Tumor-Immune microenvironment in triple negative breast cancer revealed by multiplexed ion beam imaging publication-title: Cell doi: 10.1016/j.cell.2018.08.039 – volume: 30 start-page: 589 year: 2019 ident: R9 article-title: Major pathologic response on biopsy (MPRbx) in patients with advanced melanoma treated with anti-PD-1: evidence for an early, on-therapy biomarker of response publication-title: Ann Oncol doi: 10.1093/annonc/mdz019 – volume: 9 year: 2018 ident: R63 article-title: Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue publication-title: Nat Commun doi: 10.1038/s41467-018-06089-1 – volume: 126 start-page: 3447 year: 2016 ident: R12 article-title: Tumor immune profiling predicts response to anti-PD-1 therapy in human melanoma publication-title: J Clin Invest doi: 10.1172/JCI87324 – volume: 61 start-page: 1184 year: 2008 ident: R31 article-title: Technical pitfalls potentially affecting diagnoses in immunohistochemistry publication-title: J Clin Pathol doi: 10.1136/jcp.2007.047720 – volume: 29 start-page: 1853 year: 2018 ident: R8 article-title: Pathologic features of response to neoadjuvant anti-PD-1 in resected non-small-cell lung carcinoma: a proposal for quantitative immune-related pathologic response criteria (irPRC) publication-title: Ann Oncol doi: 10.1093/annonc/mdy218 – volume: 63 start-page: 1812 year: 2016 ident: R51 article-title: Patch-Based nonlinear image registration for Gigapixel whole slide images publication-title: IEEE Trans Biomed Eng doi: 10.1109/TBME.2015.2503122 – volume: 138 start-page: 1432 year: 2014 ident: R38 article-title: Principles of analytic validation of immunohistochemical assays: guideline from the College of American pathologists pathology and laboratory quality center publication-title: Arch Pathol Lab Med doi: 10.5858/arpa.2013-0610-CP – volume: 25 start-page: 5503 year: 2019 ident: R68 article-title: High-Plex predictive marker discovery for melanoma Immunotherapy-Treated patients using digital spatial profiling publication-title: Clin Cancer Res doi: 10.1158/1078-0432.CCR-19-0104 – volume: 57 start-page: 567 year: 2009 ident: R43 article-title: Antibody elution method for multiple immunohistochemistry on primary antibodies raised in the same species and of the same subtype publication-title: J Histochem Cytochem. doi: 10.1369/jhc.2009.953240 – volume: 515 start-page: 568 year: 2014 ident: R3 article-title: PD-1 blockade induces responses by inhibiting adaptive immune resistance publication-title: Nature doi: 10.1038/nature13954 – volume: 59 start-page: 13 year: 2011 ident: R27 article-title: Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades publication-title: J Histochem Cytochem doi: 10.1369/jhc.2010.957191 – volume: 17 start-page: 95 year: 2017 ident: R66 article-title: NanoString, a novel digital color-coded barcode technology: current and future applications in molecular diagnostics publication-title: Expert Rev Mol Diagn doi: 10.1080/14737159.2017.1268533 – start-page: 42CFR493.1273(a) year: 2003 ident: R26 article-title: Clinical laboratory improvement amedments of 1988; final rule publication-title: Fed Register – volume: 3 start-page: 47 year: 2015 ident: R18 article-title: Multispectral imaging of formalin-fixed tissue predicts ability to generate tumor-infiltrating lymphocytes from melanoma publication-title: J Immunother Cancer doi: 10.1186/s40425-015-0091-z – volume: 19 start-page: 203 year: 2017 ident: R45 article-title: Quantitative multiplex immunohistochemistry reveals Myeloid-Inflamed Tumor-Immune complexity associated with poor prognosis publication-title: Cell Rep doi: 10.1016/j.celrep.2017.03.037 – volume: 465 start-page: 161 year: 2003 ident: R22 article-title: Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry publication-title: J Comp Neurol doi: 10.1002/cne.10858 – volume: 4 start-page: 76 year: 2016 ident: R21 article-title: Validation of biomarkers to predict response to immunotherapy in cancer: Volume I - pre-analytical and analytical validation publication-title: J Immunother Cancer doi: 10.1186/s40425-016-0178-1 – volume: 97 start-page: 1808 year: 2005 ident: R35 article-title: Automated quantitative analysis (aqua) of in situ protein expression, antibody concentration, and prognosis publication-title: J Natl Cancer Inst doi: 10.1093/jnci/dji427 – volume: 12 start-page: 453 year: 2015 ident: R15 article-title: Robust enumeration of cell subsets from tissue expression profiles publication-title: Nat Methods doi: 10.1038/nmeth.3337 – volume: 91 start-page: 160 year: 2017 ident: R47 article-title: Imaging mass cytometry publication-title: Cytometry Part A doi: 10.1002/cyto.a.23053 – volume: 2 start-page: 1150 year: 1999 ident: R48 article-title: Object recognition from local scale-invariant features publication-title: Proceedings of the seventh IEEE International Conference on computer vision, Kerkyra, Greece doi: 10.5555/850924.851523 – volume: 51 start-page: 1169 year: 2003 ident: R65 article-title: Double Autoimmunostaining with glycine treatment publication-title: J Histochem Cytochem. doi: 10.1177/002215540305100907 – volume: 6 start-page: 827 year: 2016 ident: R7 article-title: Analysis of immune signatures in longitudinal tumor samples yields insight into biomarkers of response and mechanisms of resistance to immune checkpoint blockade publication-title: Cancer Discov doi: 10.1158/2159-8290.CD-15-1545 – volume: 152 start-page: 75 year: 1998 ident: R53 article-title: The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin publication-title: Am J Pathol – volume: 110 start-page: 11982 year: 2013 ident: R56 article-title: Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1300136110 – volume: 2018 start-page: 105 year: 1783 ident: R67 article-title: Multiplexed Detection of RNA and Protein on the NanoString((R)) nCounter((R)) Platform publication-title: Methods Mol Biol – volume: 54 start-page: 961 year: 2006 ident: R29 article-title: Heat-Induced antigen retrieval: what are we retrieving? publication-title: J Histochem Cytochem doi: 10.1369/jhc.6P6945.2006 – volume: 5 start-page: eaax5851 year: 2019 ident: R64 article-title: MIBI-TOF: a multiplexed imaging platform relates cellular phenotypes and tissue structure publication-title: Sci Adv doi: 10.1126/sciadv.aax5851 – volume: 79 start-page: LB-318 year: 2019 ident: R58 article-title: Abstract LB-318: multi-institutional TSA-amplified multiplexed immunofluorescence reproducibility evaluation (MITRE study): reproducibility assessment of an automated multiplexed immunofluorescence slide staining, imaging, and analysis workflow publication-title: Cancer Research – volume: 84 start-page: 207 year: 2009 ident: R30 article-title: Molecular mechanisms of antigen retrieval: antigen retrieval reverses steric interference caused by formalin-induced cross-links publication-title: Biotech Histochem doi: 10.3109/10520290903039078 – volume: 48 start-page: 812 year: 2018 ident: R13 article-title: The immune landscape of cancer publication-title: Immunity doi: 10.1016/j.immuni.2018.03.023 – volume: 97 start-page: 329 year: 2017 ident: R34 article-title: Proof of the quantitative potential of immunofluorescence by mass spectrometry publication-title: Lab Invest doi: 10.1038/labinvest.2016.148 – volume: 29 start-page: 755 year: 2019 ident: R62 article-title: A map of human type 1 diabetes progression by imaging mass cytometry publication-title: Cell Metab doi: 10.1016/j.cmet.2018.11.014 – volume: 170 start-page: 1109 year: 2017 ident: R10 article-title: Oncolytic virotherapy promotes intratumoral T cell infiltration and improves anti-PD-1 immunotherapy publication-title: Cell doi: 10.1016/j.cell.2017.08.027 – volume: 1 year: 2016 ident: R41 article-title: In-Depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide publication-title: Sci Immunol doi: 10.1126/sciimmunol.aaf6925 – volume: 11 start-page: 417 year: 2014 ident: R60 article-title: Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry publication-title: Nat Methods doi: 10.1038/nmeth.2869 – volume: 10 start-page: 441 year: 2013 ident: R33 article-title: Tissue microarrays: leaping the gap between research and clinical adoption publication-title: Per Med doi: 10.2217/pme.13.42 – volume: 16 year: 2016 ident: R16 article-title: Conservation of immune gene signatures in solid tumors and prognostic implications publication-title: BMC Cancer doi: 10.1186/s12885-016-2948-z – volume: 48 start-page: 197 year: 2010 ident: R24 article-title: Antibody validation publication-title: Biotechniques doi: 10.2144/000113382 – volume: 133 start-page: 743 year: 2009 article-title: Recommended principles and practices for validating clinical molecular pathology tests publication-title: Arch Pathol Lab Med doi: 10.1043/1543-2165-133.5.743 – start-page: 42CFR493.1273(a) year: 2003 article-title: Clinical laboratory improvement amedments of 1988; final rule publication-title: Fed Register – volume: 21 start-page: 3798 year: 2003 article-title: Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis publication-title: J Clin Oncol doi: 10.1200/JCO.2003.11.069 – volume: 7 year: 2017 article-title: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues publication-title: Sci Rep doi: 10.1038/s41598-017-13942-8 – volume: 174 start-page: 968 year: 2018 article-title: Deep profiling of mouse splenic architecture with Codex multiplexed imaging publication-title: Cell doi: 10.1016/j.cell.2018.07.010 – volume: 3 start-page: 47 year: 2015 article-title: Multispectral imaging of formalin-fixed tissue predicts ability to generate tumor-infiltrating lymphocytes from melanoma publication-title: J Immunother Cancer doi: 10.1186/s40425-015-0091-z – volume: 465 start-page: 161 year: 2003 article-title: Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry publication-title: J Comp Neurol doi: 10.1002/cne.10858 – volume: 2018 start-page: 105 year: 1783 article-title: Multiplexed Detection of RNA and Protein on the NanoString((R)) nCounter((R)) Platform publication-title: Methods Mol Biol – volume: 97 start-page: 329 year: 2017 article-title: Proof of the quantitative potential of immunofluorescence by mass spectrometry publication-title: Lab Invest doi: 10.1038/labinvest.2016.148 – volume: 19 start-page: 203 year: 2017 article-title: Quantitative multiplex immunohistochemistry reveals Myeloid-Inflamed Tumor-Immune complexity associated with poor prognosis publication-title: Cell Rep doi: 10.1016/j.celrep.2017.03.037 – volume: 4 start-page: 76 year: 2016 article-title: Validation of biomarkers to predict response to immunotherapy in cancer: Volume I - pre-analytical and analytical validation publication-title: J Immunother Cancer doi: 10.1186/s40425-016-0178-1 – volume: 9 year: 2018 article-title: Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue publication-title: Nat Commun doi: 10.1038/s41467-018-06089-1 – volume: 65 start-page: 185 year: 2014 article-title: Immune modulation in cancer with antibodies publication-title: Annu Rev Med doi: 10.1146/annurev-med-092012-112807 – year: 2019 article-title: Comparison of Biomarker Modalities for Predicting Response to PD-(L)1 Checkpoint Blockade: A Systematic Review and Meta-Analysisin press publication-title: JAMA Oncol doi: 10.1001/jamaoncol.2019.1549 – volume: 57 start-page: 567 year: 2009 article-title: Antibody elution method for multiple immunohistochemistry on primary antibodies raised in the same species and of the same subtype publication-title: J Histochem Cytochem. doi: 10.1369/jhc.2009.953240 – volume: 7 year: 2012 article-title: TrakEM2 software for neural circuit reconstruction publication-title: PLoS One doi: 10.1371/journal.pone.0038011 – volume: 97 start-page: 1808 year: 2005 article-title: Automated quantitative analysis (aqua) of in situ protein expression, antibody concentration, and prognosis publication-title: J Natl Cancer Inst doi: 10.1093/jnci/dji427 – volume: 42 start-page: 433 year: 1994 article-title: F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence publication-title: J Histochem Cytochem. doi: 10.1177/42.3.7508473 – volume: 41 start-page: 141 year: 2007 article-title: Heat-Induced antigen retrieval: mechanisms and application to histochemistry publication-title: Prog Histochem Cytochem doi: 10.1016/j.proghi.2006.09.001 – volume: 54 start-page: 961 year: 2006 article-title: Heat-Induced antigen retrieval: what are we retrieving? publication-title: J Histochem Cytochem doi: 10.1369/jhc.6P6945.2006 – volume: 9 start-page: 676 year: 2012 article-title: Fiji: an open-source platform for biological-image analysis publication-title: Nat Methods doi: 10.1038/nmeth.2019 – volume: 31 start-page: 214 year: 2018 article-title: Implications of the tumor immune microenvironment for staging and therapeutics publication-title: Mod Pathol doi: 10.1038/modpathol.2017.156 – volume: 19 start-page: 982 year: 2010 article-title: Analytic variability in immunohistochemistry biomarker studies. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology publication-title: Cancer Epidemiol Biomarkers Prev doi: 10.1158/1055-9965.EPI-10-0097 – year: 2019 article-title: Automated multiplex immunofluorescence panel for Immuno-oncology studies on formalin-fixed carcinoma tissue specimens publication-title: J Vis Exp doi: 10.3791/58390 – volume: 174 start-page: 1373 year: 2018 article-title: A structured Tumor-Immune microenvironment in triple negative breast cancer revealed by multiplexed ion beam imaging publication-title: Cell doi: 10.1016/j.cell.2018.08.039 – volume: 48 start-page: 197 year: 2010 article-title: Antibody validation publication-title: Biotechniques doi: 10.2144/000113382 – volume: 51 start-page: 1169 year: 2003 article-title: Double Autoimmunostaining with glycine treatment publication-title: J Histochem Cytochem. doi: 10.1177/002215540305100907 – volume: 15 start-page: 124 year: 2007 article-title: Recommendations for improved standardization of immunohistochemistry publication-title: Appl Immunohistochem Mol Morphol doi: 10.1097/PAI.0b013e31804c7283 – volume: 5 start-page: 44 year: 2017 article-title: Identifying baseline immune-related biomarkers to predict clinical outcome of immunotherapy publication-title: J Immunother Cancer doi: 10.1186/s40425-017-0243-4 – volume: 17 start-page: 95 year: 2017 article-title: NanoString, a novel digital color-coded barcode technology: current and future applications in molecular diagnostics publication-title: Expert Rev Mol Diagn doi: 10.1080/14737159.2017.1268533 – volume: 20 start-page: 436 year: 2014 article-title: Multiplexed ion beam imaging of human breast tumors publication-title: Nat Med doi: 10.1038/nm.3488 – volume: 61 start-page: 1184 year: 2008 article-title: Technical pitfalls potentially affecting diagnoses in immunohistochemistry publication-title: J Clin Pathol doi: 10.1136/jcp.2007.047720 – volume: 152 start-page: 75 year: 1998 article-title: The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin publication-title: Am J Pathol – volume: 138 start-page: 1432 year: 2014 article-title: Principles of analytic validation of immunohistochemical assays: guideline from the College of American pathologists pathology and laboratory quality center publication-title: Arch Pathol Lab Med doi: 10.5858/arpa.2013-0610-CP – volume: 63 start-page: 1812 year: 2016 article-title: Patch-Based nonlinear image registration for Gigapixel whole slide images publication-title: IEEE Trans Biomed Eng doi: 10.1109/TBME.2015.2503122 – volume: 12 start-page: 453 year: 2015 article-title: Robust enumeration of cell subsets from tissue expression profiles publication-title: Nat Methods doi: 10.1038/nmeth.3337 – volume: 79 start-page: LB-318 year: 2019 article-title: Abstract LB-318: multi-institutional TSA-amplified multiplexed immunofluorescence reproducibility evaluation (MITRE study): reproducibility assessment of an automated multiplexed immunofluorescence slide staining, imaging, and analysis workflow publication-title: Cancer Research – volume: 493 start-page: 477 year: 2005 article-title: An open letter to our readers on the use of antibodies publication-title: J Comp Neurol doi: 10.1002/cne.20839 – volume: 6 start-page: 827 year: 2016 article-title: Analysis of immune signatures in longitudinal tumor samples yields insight into biomarkers of response and mechanisms of resistance to immune checkpoint blockade publication-title: Cancer Discov doi: 10.1158/2159-8290.CD-15-1545 – volume: 48 start-page: 812 year: 2018 article-title: The immune landscape of cancer publication-title: Immunity doi: 10.1016/j.immuni.2018.03.023 – volume: 2 start-page: 1150 year: 1999 article-title: Object recognition from local scale-invariant features publication-title: Proceedings of the seventh IEEE International Conference on computer vision, Kerkyra, Greece doi: 10.5555/850924.851523 – volume: 2020 start-page: 497 year: 2055 article-title: Multiplexed immunohistochemical consecutive staining on single slide (MICSSS): multiplexed chromogenic IHC assay for high-dimensional tissue analysis publication-title: Methods Mol Biol – volume: 515 start-page: 563 year: 2014 article-title: Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients publication-title: Nature doi: 10.1038/nature14011 – volume: 16 year: 2016 article-title: Conservation of immune gene signatures in solid tumors and prognostic implications publication-title: BMC Cancer doi: 10.1186/s12885-016-2948-z – volume: 170 start-page: 1109 year: 2017 article-title: Oncolytic virotherapy promotes intratumoral T cell infiltration and improves anti-PD-1 immunotherapy publication-title: Cell doi: 10.1016/j.cell.2017.08.027 – volume: 126 start-page: 3447 year: 2016 article-title: Tumor immune profiling predicts response to anti-PD-1 therapy in human melanoma publication-title: J Clin Invest doi: 10.1172/JCI87324 – volume: 69A start-page: 735 year: 2006 article-title: Spectral imaging: principles and applications publication-title: Cytometry Part A doi: 10.1002/cyto.a.20311 – volume: 29 start-page: 755 year: 2019 article-title: A map of human type 1 diabetes progression by imaging mass cytometry publication-title: Cell Metab doi: 10.1016/j.cmet.2018.11.014 – volume: 113 start-page: E7769 year: 2016 article-title: Association of PD-1/PD-L axis expression with cytolytic activity, mutational load, and prognosis in melanoma and other solid tumors publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1607836113 – volume: 11 start-page: 417 year: 2014 article-title: Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry publication-title: Nat Methods doi: 10.1038/nmeth.2869 – volume: 25 start-page: 470 year: 2019 article-title: Neoadjuvant nivolumab modifies the tumor immune microenvironment in resectable glioblastoma publication-title: Nat Med doi: 10.1038/s41591-018-0339-5 – volume: 25 start-page: 5503 year: 2019 article-title: High-Plex predictive marker discovery for melanoma Immunotherapy-Treated patients using digital spatial profiling publication-title: Clin Cancer Res doi: 10.1158/1078-0432.CCR-19-0104 – volume: 29 start-page: 1853 year: 2018 article-title: Pathologic features of response to neoadjuvant anti-PD-1 in resected non-small-cell lung carcinoma: a proposal for quantitative immune-related pathologic response criteria (irPRC) publication-title: Ann Oncol doi: 10.1093/annonc/mdy218 – volume: 84 start-page: 207 year: 2009 article-title: Molecular mechanisms of antigen retrieval: antigen retrieval reverses steric interference caused by formalin-induced cross-links publication-title: Biotech Histochem doi: 10.3109/10520290903039078 – volume: 110 start-page: 11982 year: 2013 article-title: Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1300136110 – volume: 515 start-page: 568 year: 2014 article-title: PD-1 blockade induces responses by inhibiting adaptive immune resistance publication-title: Nature doi: 10.1038/nature13954 – volume: 70 start-page: 46 year: 2014 article-title: Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis publication-title: Methods doi: 10.1016/j.ymeth.2014.08.016 – volume: 7 year: 2018 article-title: Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes publication-title: eLife doi: 10.7554/eLife.31657 – volume: 30 start-page: 589 year: 2019 article-title: Major pathologic response on biopsy (MPRbx) in patients with advanced melanoma treated with anti-PD-1: evidence for an early, on-therapy biomarker of response publication-title: Ann Oncol doi: 10.1093/annonc/mdz019 – volume: 10 start-page: 441 year: 2013 article-title: Tissue microarrays: leaping the gap between research and clinical adoption publication-title: Per Med doi: 10.2217/pme.13.42 – volume: 165 start-page: 780 year: 2016 article-title: Mass cytometry: single cells, many features publication-title: Cell doi: 10.1016/j.cell.2016.04.019 – volume: 91 start-page: 160 year: 2017 article-title: Imaging mass cytometry publication-title: Cytometry Part A doi: 10.1002/cyto.a.23053 – volume: 1 year: 2016 article-title: In-Depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide publication-title: Sci Immunol doi: 10.1126/sciimmunol.aaf6925 – volume: 5 start-page: eaax5851 year: 2019 article-title: MIBI-TOF: a multiplexed imaging platform relates cellular phenotypes and tissue structure publication-title: Sci Adv doi: 10.1126/sciadv.aax5851 – volume: 13 start-page: 823 year: 2016 article-title: A proposal for validation of antibodies publication-title: Nat Methods doi: 10.1038/nmeth.3995 – volume: 59 start-page: 13 year: 2011 article-title: Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades publication-title: J Histochem Cytochem doi: 10.1369/jhc.2010.957191 – ident: 2024110222451197000_8.1.e000155.37 doi: 10.1043/1543-2165-133.5.743 – volume: 152 start-page: 75 year: 1998 ident: 2024110222451197000_8.1.e000155.53 article-title: The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin publication-title: Am J Pathol – ident: 2024110222451197000_8.1.e000155.18 doi: 10.1186/s40425-015-0091-z – ident: 2024110222451197000_8.1.e000155.39 – volume: 2018 start-page: 105 year: 1783 ident: 2024110222451197000_8.1.e000155.67 article-title: Multiplexed Detection of RNA and Protein on the NanoString((R)) nCounter((R)) Platform publication-title: Methods Mol Biol – ident: 2024110222451197000_8.1.e000155.61 doi: 10.1016/j.cell.2018.08.039 – ident: 2024110222451197000_8.1.e000155.8 doi: 10.1093/annonc/mdy218 – volume: 2 start-page: 1150 year: 1999 ident: 2024110222451197000_8.1.e000155.48 article-title: Object recognition from local scale-invariant features publication-title: Proceedings of the seventh IEEE International Conference on computer vision, Kerkyra, Greece doi: 10.1109/ICCV.1999.790410 – ident: 2024110222451197000_8.1.e000155.2 doi: 10.1038/nature14011 – ident: 2024110222451197000_8.1.e000155.22 doi: 10.1002/cne.10858 – ident: 2024110222451197000_8.1.e000155.29 doi: 10.1369/jhc.6P6945.2006 – ident: 2024110222451197000_8.1.e000155.58 doi: 10.1158/1538-7445.SABCS18-LB-318 – ident: 2024110222451197000_8.1.e000155.25 doi: 10.1038/nmeth.3995 – ident: 2024110222451197000_8.1.e000155.32 doi: 10.1158/1055-9965.EPI-10-0097 – ident: 2024110222451197000_8.1.e000155.6 doi: 10.1038/s41591-018-0339-5 – volume: 91 start-page: 160 year: 2017 ident: 2024110222451197000_8.1.e000155.47 article-title: Imaging mass cytometry publication-title: Cytometry Part A doi: 10.1002/cyto.a.23053 – ident: 2024110222451197000_8.1.e000155.54 doi: 10.1016/j.cell.2018.07.010 – ident: 2024110222451197000_8.1.e000155.23 doi: 10.1002/cne.20839 – volume: 7 year: 2012 ident: 2024110222451197000_8.1.e000155.49 article-title: TrakEM2 software for neural circuit reconstruction publication-title: PLoS One doi: 10.1371/journal.pone.0038011 – ident: 2024110222451197000_8.1.e000155.4 doi: 10.1038/modpathol.2017.156 – ident: 2024110222451197000_8.1.e000155.31 doi: 10.1136/jcp.2007.047720 – ident: 2024110222451197000_8.1.e000155.24 doi: 10.2144/000113382 – ident: 2024110222451197000_8.1.e000155.1 doi: 10.1146/annurev-med-092012-112807 – ident: 2024110222451197000_8.1.e000155.10 doi: 10.1016/j.cell.2017.08.027 – ident: 2024110222451197000_8.1.e000155.55 doi: 10.7554/eLife.31657 – ident: 2024110222451197000_8.1.e000155.20 doi: 10.1016/j.cell.2016.04.019 – ident: 2024110222451197000_8.1.e000155.34 doi: 10.1038/labinvest.2016.148 – ident: 2024110222451197000_8.1.e000155.15 doi: 10.1038/nmeth.3337 – ident: 2024110222451197000_8.1.e000155.56 doi: 10.1073/pnas.1300136110 – ident: 2024110222451197000_8.1.e000155.36 doi: 10.1097/PAI.0b013e31804c7283 – volume: 16 year: 2016 ident: 2024110222451197000_8.1.e000155.16 article-title: Conservation of immune gene signatures in solid tumors and prognostic implications publication-title: BMC Cancer doi: 10.1186/s12885-016-2948-z – ident: 2024110222451197000_8.1.e000155.7 doi: 10.1158/2159-8290.CD-15-1545 – ident: 2024110222451197000_8.1.e000155.28 doi: 10.1016/j.proghi.2006.09.001 – ident: 2024110222451197000_8.1.e000155.44 doi: 10.1177/42.3.7508473 – ident: 2024110222451197000_8.1.e000155.19 doi: 10.1016/j.ymeth.2014.08.016 – ident: 2024110222451197000_8.1.e000155.27 doi: 10.1369/jhc.2010.957191 – ident: 2024110222451197000_8.1.e000155.52 doi: 10.1137/1.9780898718843 – volume: 7 year: 2017 ident: 2024110222451197000_8.1.e000155.5 article-title: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues publication-title: Sci Rep doi: 10.1038/s41598-017-13942-8 – ident: 2024110222451197000_8.1.e000155.11 doi: 10.1186/s40425-017-0243-4 – volume: 1 year: 2016 ident: 2024110222451197000_8.1.e000155.41 article-title: In-Depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide publication-title: Sci Immunol doi: 10.1126/sciimmunol.aaf6925 – ident: 2024110222451197000_8.1.e000155.43 doi: 10.1369/jhc.2009.953240 – ident: 2024110222451197000_8.1.e000155.38 doi: 10.5858/arpa.2013-0610-CP – ident: 2024110222451197000_8.1.e000155.3 doi: 10.1038/nature13954 – ident: 2024110222451197000_8.1.e000155.60 doi: 10.1038/nmeth.2869 – ident: 2024110222451197000_8.1.e000155.17 doi: 10.1001/jamaoncol.2019.1549 – volume: 10 start-page: 441 year: 2013 ident: 2024110222451197000_8.1.e000155.33 article-title: Tissue microarrays: leaping the gap between research and clinical adoption publication-title: Per Med doi: 10.2217/pme.13.42 – ident: 2024110222451197000_8.1.e000155.50 doi: 10.1038/nmeth.2019 – ident: #cr-split#-2024110222451197000_8.1.e000155.26.1 – ident: 2024110222451197000_8.1.e000155.12 doi: 10.1172/JCI87324 – ident: 2024110222451197000_8.1.e000155.68 doi: 10.1158/1078-0432.CCR-19-0104 – volume: 63 start-page: 1812 year: 2016 ident: 2024110222451197000_8.1.e000155.51 article-title: Patch-Based nonlinear image registration for Gigapixel whole slide images publication-title: IEEE Trans Biomed Eng doi: 10.1109/TBME.2015.2503122 – ident: 2024110222451197000_8.1.e000155.30 doi: 10.3109/10520290903039078 – volume: 17 start-page: 95 year: 2017 ident: 2024110222451197000_8.1.e000155.66 article-title: NanoString, a novel digital color-coded barcode technology: current and future applications in molecular diagnostics publication-title: Expert Rev Mol Diagn doi: 10.1080/14737159.2017.1268533 – volume: 30 start-page: 589 year: 2019 ident: 2024110222451197000_8.1.e000155.9 article-title: Major pathologic response on biopsy (MPRbx) in patients with advanced melanoma treated with anti-PD-1: evidence for an early, on-therapy biomarker of response publication-title: Ann Oncol doi: 10.1093/annonc/mdz019 – ident: 2024110222451197000_8.1.e000155.40 doi: 10.1200/JCO.2003.11.069 – ident: 2024110222451197000_8.1.e000155.13 doi: 10.1016/j.immuni.2018.03.023 – ident: 2024110222451197000_8.1.e000155.14 doi: 10.1073/pnas.1607836113 – volume: 19 start-page: 203 year: 2017 ident: 2024110222451197000_8.1.e000155.45 article-title: Quantitative multiplex immunohistochemistry reveals Myeloid-Inflamed Tumor-Immune complexity associated with poor prognosis publication-title: Cell Rep doi: 10.1016/j.celrep.2017.03.037 – volume: 9 year: 2018 ident: 2024110222451197000_8.1.e000155.63 article-title: Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue publication-title: Nat Commun doi: 10.1038/s41467-018-06089-1 – ident: 2024110222451197000_8.1.e000155.62 doi: 10.1016/j.cmet.2018.11.014 – volume: 69A start-page: 735 year: 2006 ident: 2024110222451197000_8.1.e000155.46 article-title: Spectral imaging: principles and applications publication-title: Cytometry Part A doi: 10.1002/cyto.a.20311 – ident: 2024110222451197000_8.1.e000155.57 doi: 10.3791/58390-v – ident: #cr-split#-2024110222451197000_8.1.e000155.26.2 – ident: 2024110222451197000_8.1.e000155.64 doi: 10.1126/sciadv.aax5851 – volume: 2020 start-page: 497 year: 2055 ident: 2024110222451197000_8.1.e000155.42 article-title: Multiplexed immunohistochemical consecutive staining on single slide (MICSSS): multiplexed chromogenic IHC assay for high-dimensional tissue analysis publication-title: Methods Mol Biol – ident: 2024110222451197000_8.1.e000155.35 doi: 10.1093/jnci/dji427 – ident: 2024110222451197000_8.1.e000155.65 doi: 10.1177/002215540305100907 – ident: 2024110222451197000_8.1.e000155.21 doi: 10.1186/s40425-016-0178-1 – ident: 2024110222451197000_8.1.e000155.59 doi: 10.1038/nm.3488 – reference: 32499296 - J Immunother Cancer. 2020 Jun;8(1):e000155corr1. doi: 10.1136/jitc-2019-000155corr1 |
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| Snippet | ObjectivesThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex... The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry... Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex... |
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| SubjectTerms | Antibodies Apoptosis Biomarkers Cancer Enzymes Fluorescent Antibody Technique - methods Gene expression Humans image analysis Immunohistochemistry - methods immunology Immunotherapy Immunotherapy - methods Mass spectrometry Microscopy oncology Peptides Position and Guidelines Position article and guidelines Proteins Scientific imaging Staining and Labeling - methods Stains & staining Tumor Microenvironment - physiology tumors |
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| Title | The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation |
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