The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation

• Published in: Journal for immunotherapy of cancer Vol. 8; no. 1; p. e000155
• Main Authors: Taube, Janis M, Akturk, Guray, Angelo, Michael, Engle, Elizabeth L, Gnjatic, Sacha, Greenbaum, Shirley, Greenwald, Noah F, Hedvat, Cyrus V, Hollmann, Travis J, Juco, Jonathan, Parra, Edwin R, Rebelatto, Marlon C, Rimm, David L, Rodriguez-Canales, Jaime, Schalper, Kurt A, Stack, Edward C, Ferreira, Cláudia S, Korski, Konstanty, Lako, Ana, Rodig, Scott J, Schenck, Emanuel, Steele, Keith E, Surace, Michael J, Tetzlaff, Michael T, von Loga, Katharina, Wistuba, Ignacio I, Bifulco, Carlo B
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd 01.05.2020; BMJ Publishing Group LTD; BMJ Publishing Group
• Subjects: Antibodies; Apoptosis; Biomarkers; Cancer; Enzymes; Fluorescent Antibody Technique - methods; Gene expression; Humans; image analysis; Immunohistochemistry - methods; immunology; Immunotherapy; Immunotherapy - methods; Mass spectrometry; Microscopy; oncology; Peptides; Position and Guidelines; Position article and guidelines; Proteins; Scientific imaging; Staining and Labeling - methods; Stains & staining; Tumor Microenvironment - physiology; tumors; tumors; image analysis; immunology; oncology
• ISSN: 2051-1426; 2051-1426
• DOI: 10.1136/jitc-2019-000155

ObjectivesThe interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.MethodsThe Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.ResultsRepresentative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.ConclusionsmIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.