Analysis of LMP and TAP polymorphisms by polymerase chain reaction-restriction fragment length polymorphism in rheumatoid arthritis

• Published in: Annals of the rheumatic diseases Vol. 57; no. 1; pp. 33 - 37
• Main Authors: Vinasco, J, Fraile, A, Nieto, A, Beraun, Y, Pareja, E, Mataran, L, Martín, J
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01.01.1998; BMJ; Elsevier Limited
• Subjects: Antigen presentation; Arthritis, Rheumatoid - genetics; ATP Binding Cassette Transporter, Subfamily B, Member 2; ATP-Binding Cassette Transporters - genetics; Autoimmune diseases; Biological and medical sciences; Cysteine Endopeptidases; Deoxyribonucleic acid; Disease; Diseases of the osteoarticular system; DNA; Extended Reports; Genes; Genes, MHC Class II; Genetic Predisposition to Disease; Humans; Hypotheses; Inflammatory joint diseases; Laboratories; large molecular weight proteasome; Medical sciences; Molecular weight; Pathogenesis; Patients; Peptides; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Proteins; Proteins - genetics; rheumatoid arthritis; Studies; transporter assoicated with antigen processing; Human; Immunopathology; Antigen presentation; Immune response; Pathogenesis; HLA-DR-System; Diseases of the osteoarticular system; Class II histocompatibility antigen; Autoimmune disease; Exploration; Inflammatory joint disease; Polymerase chain reaction; Chronic; Restriction fragment length polymorphism; Rheumatoid arthritis; T-Lymphocyte; Genetics; Molecular biology; Polymorphism
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.57.1.33

OBJECTIVE The aim of this study was to investigate the relation between the polymorphism of large molecular weight proteasome (LMP) (LMP2-LMP7) and transporter associated with antigen processing (TAP) (TAP1-TAP2) genes and rheumatoid arthritis (RA). METHODS Sixty RA patients and 102 ethnically matched unrelated healthy subjects were typed for LMP, TAP, and disease associated HLA-DRB1 alleles by using a new strategy based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with amplification created restriction sites. RESULTS The polymorphism of LMP (LMP2-LMP7) and TAP (TAP1-TAP2) genes was examined in shared epitope positive and negative RA patients and controls. No significant differences in the LMP or TAP allele frequencies were observed between the total patient and control groups or the patients and controls positive or negative for the shared epitope. CONCLUSION The data suggest that the polymorphisms of LMP and TAP genes do not have an important influence in the pathogenesis of RA, although larger studies will be needed to provide more conclusive evidence on the role of these genes in RA. A new, highly reliable strategy for typing LMP alleles is also described.