Masters of Misdirection: Peptidoglycan Glycosidases in Bacterial Growth

• Published in: Journal of bacteriology Vol. 205; no. 3; p. e0042822
• Main Authors: Weaver, Anna, Taguchi, Atsushi, Dörr, Tobias
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 21.03.2023
• Subjects: Autolysins; Bacteria; Bacteria - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Cell Division; Cell Wall - metabolism; Cell walls; Enzymes; Glycan; Glycosidases; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Localization; Minireview; Penicillin; Peptidoglycan - metabolism; Peptidoglycans; Physiology; Protein interaction; Proteins; Redundancy; Special Series: 2022 Molecular Genetics of Bacteria and Phages Meeting; Strands; Substrate preferences; Substrates; glycosidase; peptidoglycan; lytic transglycosylase; glucosaminidase; muramidase; peptidoglycan hydrolases
• ISSN: 0021-9193; 1098-5530; 1098-5530
• DOI: 10.1128/jb.00428-22

The dynamic composition of the peptidoglycan cell wall has been the subject of intense research for decades, yet how bacteria coordinate the synthesis of new peptidoglycan with the turnover and remodeling of existing peptidoglycan remains elusive. Diversity and redundancy within peptidoglycan synthases and peptidoglycan autolysins, enzymes that degrade peptidoglycan, have often made it challenging to assign physiological roles to individual enzymes and determine how those activities are regulated. The dynamic composition of the peptidoglycan cell wall has been the subject of intense research for decades, yet how bacteria coordinate the synthesis of new peptidoglycan with the turnover and remodeling of existing peptidoglycan remains elusive. Diversity and redundancy within peptidoglycan synthases and peptidoglycan autolysins, enzymes that degrade peptidoglycan, have often made it challenging to assign physiological roles to individual enzymes and determine how those activities are regulated. For these reasons, peptidoglycan glycosidases, which cleave within the glycan strands of peptidoglycan, have proven veritable masters of misdirection over the years. Unlike many of the broadly conserved peptidoglycan synthetic complexes, diverse bacteria can employ unrelated glycosidases to achieve the same physiological outcome. Additionally, although the mechanisms of action for many individual enzymes have been characterized, apparent conserved homologs in other organisms can exhibit an entirely different biochemistry. This flexibility has been recently demonstrated in the context of three functions critical to vegetative growth: (i) release of newly synthesized peptidoglycan strands from their membrane anchors, (ii) processing of peptidoglycan turned over during cell wall expansion, and (iii) removal of peptidoglycan fragments that interfere with daughter cell separation during cell division. Finally, the regulation of glycosidase activity during these cell processes may be a cumulation of many factors, including protein-protein interactions, intrinsic substrate preferences, substrate availability, and subcellular localization. Understanding the true scope of peptidoglycan glycosidase activity will require the exploration of enzymes from diverse organisms with equally diverse growth and division strategies.