Super-Resolved Traction Force Microscopy (STFM)

Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater th...

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Bibliographic Details
Published inNano letters Vol. 16; no. 4; pp. 2633 - 2638
Main Authors Colin-York, Huw, Shrestha, Dilip, Felce, James H, Waithe, Dominic, Moeendarbary, Emad, Davis, Simon J, Eggeling, Christian, Fritzsche, Marco
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 13.04.2016
Subjects
Algorithms
Biology
Cell Adhesion
Computer Simulation
Letter
Microscopy
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Microscopy, Scanning Probe - instrumentation
Microscopy, Scanning Probe - methods
Models, Theoretical
Nanostructure
Reconstruction
Sampling
Spatial resolution
Stress, Mechanical
Traction
Traction force
Super-resolution microscopy
mechanobiology
actin cytoskeleton
traction force microscopy
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ISSN1530-6984
1530-6992
1530-6992
DOI10.1021/acs.nanolett.6b00273

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Summary:Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
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ISSN:1530-6984
1530-6992
1530-6992
DOI:10.1021/acs.nanolett.6b00273