Glucose-6-Phosphate-Functionalized Magnetic Microsphere as Novel Hydrophilic Probe for Specific Capture of N‑Linked Glycopeptides

• Published in: Analytical chemistry (Washington) Vol. 89; no. 20; pp. 11151 - 11158
• Main Authors: Li, Yilin, Wang, Jiawen, Sun, Nianrong, Deng, Chun-hui
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.10.2017
• Subjects: Analytical chemistry; animals; Antibiotics; Biocompatibility; blood serum; Carbohydrates; Chemistry; Chromatography; Chromatography, High Pressure Liquid; cost effectiveness; Enrichment; Fabrication; Ferrosoferric Oxide - chemistry; Functional groups; Glucose; Glucose-6-phosphate; Glucose-6-Phosphate - chemistry; Glycopeptides; Glycopeptides - analysis; Glycoproteins; Horseradish Peroxidase - metabolism; Humans; hydrophilic interaction chromatography; hydrophilicity; Hydrophobic and Hydrophilic Interactions; Immunoglobulin G - metabolism; Iron oxides; Liquid chromatography; Magnetics; Microspheres; Peptides; Saliva; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.7b03708

Developing cost-effective approaches based on hydrophilic interaction liquid chromatography (HILIC) has been the main tendency for low-abundance glycopeptides capture before LC-MS/MS analysis. Carbohydrates with outstanding biocompatibility and hydrophilicity are ubiquitous in the kingdoms of animal and plant and could be a wonderful choice as functional groups for glycopeptides enrichment. In this work, glucose-6-phosphate, as one of the indispensable cogs in pivotal metabolic wheels of life, was chosen as functionalized groups to be grafted onto the surface of Fe3O4 microspheres via one-step surface fabrication strategy. The acquired hydrophilic Fe3O4@G6P microspheres showed superior enrichment performance for glycopeptides with high sensitivity (0.5 fmol/μL) and high selectivity (1:100) and good repeatability (10 times at least). Furthermore, the Fe3O4@G6P microspheres also exhibited enrichment ability for glycopeptides in different biosamples. A total of 243 glycopeptides assigned to 92 glycoproteins and 183 glycopeptides corresponding to 74 different glycoproteins was identified from merely 2 μL of serum and saliva, respectively.