Array-Based Disease Diagnostics Using Lipid/Polydiacetylene Vesicles Encapsulated in a Sol–Gel Matrix

• Published in: Analytical chemistry (Washington) Vol. 84; no. 14; pp. 5925 - 5931
• Main Authors: Kolusheva, S, Yossef, R, Kugel, A, Katz, M, Volinsky, R, Welt, M, Hadad, U, Drory, V, Kliger, M, Rubin, E, Porgador, A, Jelinek, R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 17.07.2012
• Subjects: Adult; Aged; Aged, 80 and over; algorithms; Amyotrophic Lateral Sclerosis - blood; Amyotrophic Lateral Sclerosis - diagnosis; Analytical chemistry; artificial intelligence; blood plasma; Capsules; Chemistry; colorimetry; Colorimetry - instrumentation; Diagnosis, Differential; diagnostic techniques; Disease; Exact sciences and technology; Female; gels; Humans; Inflammatory Bowel Diseases - blood; Inflammatory Bowel Diseases - diagnosis; lipid composition; Lipids; Lipids - chemistry; Male; Matrix; Medical diagnosis; Medical screening; Metabolites; Middle Aged; patients; Polyacetylenes - chemistry; Polymers - chemistry; screening; Silica; silica gel; Silica Gel - chemistry; Spectrometric and optical methods; Young Adult; Human; Metabolite; Lipids; Colorimetry; Amphiphilic compound; Blood plasma; Array; Screening; Sol gel process; Fingerprint method; Membrane; Silica gel; Diagnosis; Learning algorithm; Application
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/ac300449u

We demonstrate a novel array-based diagnostic platform comprising lipid/polydiacetylene (PDA) vesicles embedded within a transparent silica-gel matrix. The diagnostic scheme is based upon the unique chromatic properties of PDA, which undergoes blue-red transformations induced by interactions with amphiphilic or membrane-active analytes. We show that constructing a gel matrix array hosting PDA vesicles with different lipid compositions and applying to blood plasma obtained from healthy individuals and from patients suffering from disease, respectively, allow distinguishing among the disease conditions through application of a simple machine-learning algorithm, using the colorimetric response of the lipid/PDA/gel matrix as the input. Importantly, the new colorimetric diagnostic approach does not require a priori knowledge on the exact metabolite compositions of the blood plasma, since the concept relies only on identifying statistically significant changes in overall disease-induced chromatic response. The chromatic lipid/PDA/gel array-based “fingerprinting” concept is generic, easy to apply, and could be implemented for varied diagnostic and screening applications.