Comparison of Streptococcus pneumoniae isolates occurring in optochin-susceptible and optochin-resistant variants by analyzing whole-genome sequencing data

• Published in: Microbiology spectrum Vol. 13; no. 4; p. e0193924
• Main Authors: Vohrnová, Sandra, Kozáková, Jana, Honskus, Michal
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2025
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Czech Republic; Drug Resistance, Bacterial - genetics; Genetics and Molecular Biology; Genome, Bacterial; Humans; Microbial Sensitivity Tests; Multilocus Sequence Typing; optochin; Pneumococcal Infections - microbiology; Quinine - analogs & derivatives; Research Article; Serotyping; Streptococcus pneumoniae; Streptococcus pneumoniae - classification; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - isolation & purification; Whole Genome Sequencing; Czech Republic; whole genome sequencing; Streptococcus pneumoniae; optochin
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.01939-24

Globally, among the most fundamental tests used for the identification of Streptococcus pneumoniae isolates is determining susceptibility to optochin. In the last 2 decades, optochin-resistant strains have been frequently reported in the literature, which can lead to the misidentification of S. pneumoniae . This study compares whole-genome sequencing data of optochin-susceptible and optochin-resistant subpopulations of S. pneumoniae isolates and investigates the genetic probable causes of resistance in the genomes of optochin-resistant subpopulations.