In Vitro and in Vivo Antioxidant and Anti-inflammatory Capacities of an Antioxidant-Rich Fruit and Berry Juice Blend. Results of a Pilot and Randomized, Double-Blinded, Placebo-Controlled, Crossover Study

• Published in: Journal of agricultural and food chemistry Vol. 56; no. 18; pp. 8326 - 8333
• Main Authors: Jensen, Gitte S, Wu, Xianli, Patterson, Kelly M, Barnes, Janelle, Carter, Steve G, Scherwitz, Larry, Beaman, Robert, Endres, John R, Schauss, Alexander G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 24.09.2008
• Subjects: administration & dosage; Adult; analysis; anthocyanins; Anthocyanins - administration & dosage; anti-inflammatory activity; Anti-Inflammatory Agents; Anti-Inflammatory Agents - administration & dosage; antioxidant activity; Antioxidants; Antioxidants - administration & dosage; Beverages; Bioactive Constituents; Biological and medical sciences; blended foods; blood; chemistry; Cross-Over Studies; Double-Blind Method; drug effects; Fish and seafood industries; Food industries; Fruit; Fruit - chemistry; Fruit and vegetable industries; fruit juices; Fundamental and applied biological sciences. Psychology; Humans; in vitro studies; in vivo studies; inflammation; lipid peroxidation; Middle Aged; Neutrophils; Neutrophils - drug effects; Pilot Projects; pilot studies; Placebos; reactive oxygen species; Reactive Oxygen Species - blood; small fruits; Thiobarbituric Acid Reactive Substances; Thiobarbituric Acid Reactive Substances - analysis; thiobarbituric acid reactive substances assay (TBARS); fruit juice; anti-inflammatory; oxygen radical absorbance capacity (ORAC) assay; cell-based antioxidant protection assay (CAP-e); Antioxidant; lipid peroxidation; açai, Euterpe oleracea; Bass; Edible fish; Oxygen; Small fruits; Antiinflammatory agent; Fruit product; Lipids; In vitro; Fruit juice; Antioxidant; anti-inflammatory; lipid peroxidation; cell-based antioxidant protection assay (CAP-e); oxygen radical absorbance capacity (ORAC) assay; acai; Assay; In vivo; Euterpe oleracea; fruit juice; thiobarbituric acid reactive substances assay (TBARS); Cell; Protection; Peroxidation
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/jf8016157

This study investigated the in vitro and in vivo antioxidant and anti-inflammatory properties of a juice blend (JB), MonaVie Active, containing a mixture of fruits and berries with known antioxidant activity, including açai, a palm fruit, as the predominant ingredient. The phytochemical antioxidants in the JB are primarily in the form of anthocyanins, predominantly cyanidin 3-rutoside, cyanidin 3-diglycoside, and cyanidin 3-glucoside. The cell-based antioxidant protection of erythrocytes (CAP-e) assay demonstrated that antioxidants in the JB penetrated and protected cells from oxidative damage (p < 0.001), whereas polymorphonuclear cells showed reduced formation of reactive oxygen species (p < 0.003) and reduced migration toward three different pro-inflammatory chemoattractants: fmlp (p < 0.001), leukotriene B4 (p < 0.05), and IL-8 (p < 0.03). A randomized, double-blinded, placebo-controlled, crossover trial with 12 healthy subjects examined the JB’s antioxidant activity in vivo. Blood samples at baseline, 1 h, and 2 h following consumption of the JB or placebo were tested for antioxidant capacity using several antioxidant assays and the TBARS assay, a measure of lipid peroxidation. A within subject comparison showed an increase in serum antioxidants at 1 h (p < 0.03) and 2 h (p < 0.015), as well as inhibition of lipid peroxidation at 2 h (p < 0.01) postconsumption.