Direct Visualization of Transient Thermal Response of a DNA Origami

The DNA origami approach enables the construction of complex objects from DNA strands. A fundamental understanding of the kinetics and thermodynamics of DNA origami assembly is extremely important for building large DNA structures with multifunctionality. Here both experimental and theoretical studi...

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Published inJournal of the American Chemical Society Vol. 134; no. 24; pp. 9844 - 9847
Main Authors Song, Jie, Arbona, Jean-Michel, Zhang, Zhao, Liu, Lei, Xie, Erqing, Elezgaray, Juan, Aime, Jean-Pierre, Gothelf, Kurt Vesterager, Besenbacher, Flemming, Dong, Mingdong
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 20.06.2012
Subjects
atomic force microscopy
DNA
DNA - chemistry
melting
Microscopy, Atomic Force
Nanostructures - chemistry
Nanostructures - ultrastructure
Nucleic Acid Conformation
Nucleic Acid Denaturation
temperature
Thermodynamics
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ISSN0002-7863
1520-5126
1520-5126
DOI10.1021/ja3017939

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Summary:The DNA origami approach enables the construction of complex objects from DNA strands. A fundamental understanding of the kinetics and thermodynamics of DNA origami assembly is extremely important for building large DNA structures with multifunctionality. Here both experimental and theoretical studies of DNA origami melting were carried out in order to reveal the reversible association/disassociation process. Furthermore, by careful control of the temperature cycling via in situ thermally controlled atomic force microscopy, the self-assembly process of a rectangular DNA origami tile was directly visualized, unveiling key mechanisms underlying their structural and thermodynamic features.
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja3017939